莫诺甙环口差向异构体的HPLC和ESI-TOF-MS分析  

HPLC and ESI-TOF-MS Analysis of the Anomeric Isomers of Morroniside

作　　者：闫存玉[1] 赵毅民[1] 乔善义[1] 刘勤[1] 谢剑炜[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《高等学校化学学报》2003年第10期1784-1786,共3页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展规划项目 (批准号 :G19990 5 44 0 1)资助

摘　　要：Morroniside consists of two anomeric isomers, α-morroniside and β-morroniside. These two anomeric compounds have been separated by reversed-phase high-performance liquid chromatography(HPLC) using a gradient mobile phase of acetonitrile-water at 7 ℃, and verified to be anomers of morroniside by ESI-TOF-MS. The higher peak in the chromatogram was appointed to α-morroniside according to NMR data reported in previous literature. The molar ratio of α-morroniside to β-morroniside was found to be 2.855∶1 at 25 ℃. Temperature appeared to be an important factor, which affects the separation result, and a better separation was achieved at a lower temperature. The relative peak height of β-morroniside in the total ion current chromatogram measured with positive ion ESI-TOF mass spectrometer was only a half of that in UV chromatogram. This indicates that β-morroniside is less sensitive to positive ion electrospray ionization than the α-isomer.Morroniside consists of two anomeric isomers, α-morroniside and β-morroniside. These two anomeric compounds have been separated by reversed-phase high-performance liquid chromatography(HPLC) using a gradient mobile phase of acetonitrile-water at 7 ℃, and verified to be anomers of morroniside by ESI-TOF-MS. The higher peak in the chromatogram was appointed to α-morroniside according to NMR data reported in previous literature. The molar ratio of α-morroniside to β-morroniside was found to be 2.855∶1 at 25 ℃. Temperature appeared to be an important factor, which affects the separation result, and a better separation was achieved at a lower temperature. The relative peak height of β-morroniside in the total ion current chromatogram measured with positive ion ESI-TOF mass spectrometer was only a half of that in UV chromatogram. This indicates that β-morroniside is less sensitive to positive ion electrospray ionization than the α-isomer.

关 键 词：莫诺甙 差向异构体 HPLC ESI-TOF-MS分析 中药 山茱萸 电喷雾质谱 色谱分析 

分 类 号：R284.2[医药卫生—中药学] R282.71[医药卫生—中医学]