中性红荧光探针法测定生物大分子核酸  

Determination of Trace Nucleic Acids Using Neutral Red as the Fluorescent Probe

作　　者：李文友[1] 吴会灵[1] 何锡文[1] 梁宏[2] 

机构地区：[1]南开大学化学系,元素有机化学国家重点实验室,天津300071 [2]广西师范大学化学系,桂林541004

出　　处：《高等学校化学学报》2003年第10期1787-1789,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 175 0 0 9);教育部博士学科点专项基金 (批准号 :2 0 0 2 0 0 5 5 0 0 2 );广西省教育厅研究项目资助

摘　　要：The fluorescence spectra and binding reaction of neutral red with nucleic acids have been studied. At pH=4.5, a new method for the determination of trace nucleic acids based on fluorescence quenching measurement was established. When 8.0×10 -6 mol/L NR is employed, the linear ranges of the calibration graphs are 0.08-2.0 μg/mL for calf thymus DNA(ctDNA), 0.12-2.2 μg/mL for fish sperm DNA(fsDNA) and 0.30-1.4 μg/mL for yeast RNA(yRNA), respectively. The corresponding detection limits are 30, 33 and 66 ng/mL, respectively. The real samples were analyzed satisfactorily.The fluorescence spectra and binding reaction of neutral red with nucleic acids have been studied. At pH=4.5, a new method for the determination of trace nucleic acids based on fluorescence quenching measurement was established. When 8.0×10 -6 mol/L NR is employed, the linear ranges of the calibration graphs are 0.08-2.0 μg/mL for calf thymus DNA(ctDNA), 0.12-2.2 μg/mL for fish sperm DNA(fsDNA) and 0.30-1.4 μg/mL for yeast RNA(yRNA), respectively. The corresponding detection limits are 30, 33 and 66 ng/mL, respectively. The real samples were analyzed satisfactorily.

关 键 词：中性红 荧光探针 测定 生物大分子 核酸 测定 DNA 

分 类 号：Q523[生物学—生物化学] Q503