宁夏鼠疫菌质粒生物学特征研究  

Biological feature of Yersinia pestis plasmid in Ningxia

作　　者：王自存[1] 赵建华[1] 张涛[1] 王富全[1] 高桂英[1] 刘兴国[1] 袁云海[1] 

机构地区：[1]宁夏疾病预防控制中心,宁夏银川750004

出　　处：《中国地方病学杂志》2003年第5期407-409,共3页Chinese Jouranl of Endemiology

摘　　要：目的　研究宁夏 12 6株鼠疫菌的质粒、毒力决定因子与毒力的关系 ,预测动物鼠疫的流行。方法　质粒凝胶电泳、毒力因子测定和半数致死量 (L D50 )试验。结果　被试菌株多数含有 6 ,13,4 5 ,6 5 MD质粒 ,具有 4种毒力因子 ;在流行末期分离的菌株毒力较弱。Objective To study relationships of the plasmid virulent factors and virulence of 126 Yersinia(Y.) pestis and predict epidemic period.Methods Agrose gel ectrophoresis detction of virulent factors and test of LD 50 .Results The straints carried 4 different kinds of plasmid with the molecular weight about 6,13,45,65 MD and 4 kinds virulent factor of Y.pestis.The virlent of the straints was weakness isolated from epidemic last stage.Conclusions The analysis of the plasmid could be used for diffrential diagnosis of Y.pestis.

关 键 词：宁夏 鼠疫菌 质粒 生物学特征 毒力 流行病学 

分 类 号：R378[医药卫生—病原生物学]