血管内皮型一氧化氮合酶基因Glu298Asp多态性与老年心肌梗死的关系  

Association of Glu298Asp polymorphism of the endothelial nitric oxide synthase gene with myocardial infarction in the elderly patients

作　　者：占伊扬[1] 狄群[1] 程蕴琳[1] 丁小建[2] 

机构地区：[1]南京医科大学第一附属医院老年医学科,210029 [2]南京医科大学第一附属医院中心实验室,210029

出　　处：《中华老年医学杂志》2003年第9期523-525,共3页Chinese Journal of Geriatrics

基　　金：江苏省自然科学资金资助项目 (编号 :BK2 0 0 1162 )

摘　　要：目的　探讨老年人血管内皮型一氧化氮合酶 (eNOS)基因Glu2 98Asp多态性的分布及其与老年人心肌梗死的关系。　方法　测定 37例老年心肌梗死患者和 172例性别、年龄相匹配对照者的身高、体重、空腹血脂等指标 ,采用聚合酶链反应 (PCR)和限制性片断长度多态性 (RFLP)检测eNOS基因Glu2 98Asp多态性。　 结果　患病组Glu/Asp基因型高于对照组 (32 4 %和 18 0 % ) ,两组eNOS基因Glu2 98Asp多态性构成差异有显著性 (χ2 =3 87,P <0 0 5 )。无高血压患病亚组Glu/Asp基因型高于无高血压对照亚组 (35 0 %和 10 9% ) ,两亚组Glu2 98Asp多态性构成差异亦有显著性 (χ2 =7 4 3,P <0 0 1)。患病组 2 98Asp等位基因频率高于对照组 (16 2 %和 9 0 % ,P >0 0 5 ) ;无高血压患病亚组 2 98Asp等位基因频率亦高于无高血压对照亚组 (17 5 %和 5 4 % ) ,两组比较差异有显著性 (χ2 =6 82 ,P <0 0 1)。　结论　eNOS基因Glu2 98Asp多态性在中国老年人群中存在 ,Glu/Asp基因型和 2 98Asp等位基因可能是中国老年人心肌梗死的遗传易感指标。Objective To explore the association of Glu298Asp polymorphism of the endothelial nitric oxide synthase gene (eNOS) with myocardial infarction in Chinese aged people based on a case-control study. Methods Thirty-seven patients with myocardial infarction and 172 sex- and age-matched healthy control subjects were enrolled. Their height, weight and blood pressure were measured and the fasting plasma lipid concentrations were examined. Glu298Asp polymorphisms of the eNOS gene were examined using PCR and RFLP methods. Results Genotype Glu/Asp in the patient group was higher than that in the control group (32.4% vs 18.0%). The genotype composition of Glu298Asp polymorphism showed significant difference between the patient group and the control group ( =3.87, P <0.05). Among normotensive subgroup, genotype Glu/Asp in the patient subgroup was higher than that in the control subgroup (35.0% vs 10.9% ). The genotype composition of Glu298Asp polymorphism of the eNOS gene also had significant difference between the patient subgroup and the control subgroup ( =7.43, P <0.01). The allelic frequencies of 298Asp in the patient group was higher than that in the control group(16.2% vs 9.0%, P >0.05). Among the normotensive subgroup, the allelic frequencies of 298Asp in the patient subgroup was higher than that in the control subgroup (17.5% vs 5.4%). The allelic frequencies of 298Asp had significant difference between the patient subgroup and the control subgroup ( =6.82, P <0.01). Conclusions Glu298Asp polymorphism of the endothelial nitric oxide synthase gene exists in Chinese aged people. The genotype Glu/Asp and allelic frequencies of 298Asp might be genetic susceptible markers of myocardial infarction.

关 键 词：老年人 心肌梗死 一氧化氮合酶 基因多态性 

分 类 号：R542.22[医药卫生—心血管疾病]