参芪方血清对慢性肾衰大鼠肾脏细胞增殖的影响  

Influence of Shenqi Recipe on mesangial cells and inter stitial fibroblasts of chronic renal failure rats

作　　者：穆静[1] 李仪奎[1] 符胜光[1] 

机构地区：[1]上海中医药大学中药新药药理毒理研究中心,上海200032

出　　处：《中西医结合学报》2003年第2期122-124,共3页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目 ( 396 30 36 0 )

摘　　要：目的　观察参芪方对大鼠肾小球系膜细胞和肾间质成纤维细胞增殖的影响。方法　应用血清药理实验方法 ,采用MTT比色法观察正常和肾衰大鼠血清对体外培养的肾小球系膜细胞和肾间质成纤维细胞增殖的影响。结果　与正常空白大鼠血清相比较 ,参芪方含药血清非常明显抑制两种细胞的增殖 (P <0 .0 0 1) ;与肾衰大鼠模型组血清相比 ,该方含药血清能够抑制两种细胞的增殖 (P <0 .0 0 1)。ObjectiveTo observe the effect of Shen qi Recipe on the proliferation of glomerular mesangial cells (MC) and interstitial fibroblasts (FC) in rats. MethodsThe effect of serum containing prepared drugs from normal and chronic renal failure rats treated by Shenqi Recipe on glomerular mesangial cells and intersititial fibroblasts in vit ro was observed by MTT colorimetric assay and serophrmacology. ResultsH TSSCompared with the blank sample normal rats, serum containing She nqi Recipe inhibited the proliferation of MC and FC(P<0.001), and the seru m containing Shenqi Recipe of CRF rats had significantly inhibited the prolifera tion of MC and FC compared with the model group(P<0.001). Conclusion Shenqi Recipe can postpone CRF progress by this mechanism.

关 键 词：参芪方 血清 慢性肾衰 大鼠 肾脏细胞增殖 肾小球系膜细胞 肾间质成纤维细胞 

分 类 号：R285.5[医药卫生—中药学] R277.525[医药卫生—中医学]