阿诺宁乳化剂的抗瘤作用和急性毒性试验  

Studies on antitumor effects and acute toxicity of anuoning emulsion

作　　者：谢冰芬[1] 冯公侃[1] 王鸿鹤[1] 朱孝峰[1] 刘宗潮[1] 杨仁洲[2] 魏孝义[2] 

机构地区：[1]中山大学肿瘤防治中心肿瘤研究所,广东广州510060 [2]中国科学院华南植物研究所,广东广州510650

出　　处：《中国药学杂志》2003年第8期590-595,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金 (3 9870 886;3 990 0 183 ) ;中山医科大学"2 11工程"重点学科 (980 69)项目

摘　　要：目的　研究阿诺宁乳化剂的抗瘤作用和急性毒性反应。方法　用小鼠和裸鼠移植性肿瘤模型测定体内抗瘤活性 ,用Blliss方法计算小鼠给药的急性毒性的LD50 。结果　阿诺宁乳化剂在 4 ,8和 16mg·kg-1,灌胃 (ig) qd× 10d ,对小鼠肝癌HepS 3批实验的平均抑瘤率分别为 39.2 % ,4 6 .9%和 5 5 .7% ;对小鼠肉瘤S 180的平均抑瘤率分别为 34.6 % ,4 7.3%和5 4 .4 % ;对小鼠L2 的平均抑瘤率分别为 37.5 % ,4 5 .9%和 5 6 .5 %。阿诺宁乳化剂在 7.5 ,15 ,30和 6 0 μg·kg-1腹腔注射 (ip)×10d剂量下 ,对小鼠肝癌 (HepS)实验的平均抑瘤率分别为 31.0 % ,4 2 .9% ,5 0 .2 %和 6 2 .4 % ;对小鼠肉瘤S 180的平均抑瘤率依次为 2 3.6 % ,39.4 % ,5 0 .9%和 6 1.2 % ;在 15 ,30和 6 0 μg·kg-1,ip ,每日 1次 ,每周 6次 ,共 4周的剂量下 ,对人肝癌 (Bel 74 0 2 )裸鼠移植瘤的抑瘤率分别为 32 .0 % ,5 0 .5 %和 6 0 .2 % ;在上述 3个剂量下 ,对人肺腺癌 (GLC 82 )裸鼠移植瘤的抑瘤率分别为 30 .3% ,4 1.9%和 5 6 .1%。结果表明 ,阿诺宁乳化剂ig ,ip对上述 5种肿瘤均有明显的抑制作用 ,且其抑瘤率与阿诺宁乳剂的剂量相关。用Bliss方法统计出阿诺宁乳化剂对小鼠ig一次的半数致死量 (LD50 )及其 95 %可信限为 74 .75 (5 8.6 7?OBJECTIVE: To investigate the antitumor effect and acute toxicity of anuoning emulsion in mice. METHODS: The models transplanted tumor in mice and nude mice were used. Blliss method was used to calculate the lethal dose of 50% (LD50) of anuoning emulsion in mice. RESULTS: Under the doses of 4,8 and 16 mg&middotkg-1 anuoning emulsion intragstic (ig), qd × 10 d, the average tumor inhibition rates were 39.2%, 46.9% and 55.7% respectively (P 2 tumor, the mean inhibition rates were 37.5%, 45.9%, and 56.5 % (P -1, intraperitoneal injection (ip) qd × 10 d to mice tumor HepS, the mean inhibition rates were 31.0% 42.9% 50.2% and 62.4% (P -1, ip × 6/w × 4 w, the inhibitory rates were 32.0%, 50.5%, and 60.2% (P 50 and its 95% confidence interval was 74.75 (58.669-95.26) mg&middotkg-1. In single ip management to mice, LD50 of anuoning emulsion was 1.12(1.01-1.24) mg&middotkg-1. In single intravenous (iv) administration LD50 was 1.81(1.61-2.02) mg&middotkg-1. CONCLUSION: The experiment results indicated that anuoning emulsion possessed obvious antitumor effects against various transplanted tumor in mice and nude mice.

关 键 词：阿诺宁乳化剂 急性毒性试验 抗瘤活性 小鼠 移植性肿瘤 

分 类 号：R979.1[医药卫生—药品] R730.53[医药卫生—药学]