核黄素的微分脉冲溶出伏安分析  

Determination of Riboflavin by Differential Pulse Adsorptive Stripping Voltammetry

作　　者：唐平[1] 王娟[2] 曾百肇[2] 

机构地区：[1]江汉大学化学与环境科学学院,武汉430056 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析科学学报》2003年第4期309-312,共4页Journal of Analytical Science

基　　金：中科院长春应化所电分析化学开放实验室基金

摘　　要：采用循环伏安法和微分脉冲溶出伏安法,对核黄素在裸金电极和巯基化合物分子自组装膜修饰金电极上的电化学行为进行了研究,发现在pH4.8的B-R缓冲溶液中,核黄素在裸金电极和分子自组装膜修饰金电极上均于-0.35V左右产生一对可逆的氧化还原峰。核黄素在裸金电极和谷胱甘肽、三巯基丙酸、二巯基苯丙咪唑分子自组装膜修饰金电极上,其浓度分别在3.0×10-7～2.3×10-4mol/L、1.05×10-6～2.0×10-4mol/L、2.1×10-6～2.08×10-4mol/L、1.05×10-6～2.0×10-4mol/L范围内与微分脉冲伏安峰峰电流之间有良好的线性关系,其相关系数分别为0.9932、0.9909、0.9857、0.9832,核黄素的检出限为2.1×10-7mol/L、5.2×10-7mol/L、8.6×10-7mol/L、5.2×10-7mol/L。对浓度为1.0×10-5mol/L的核黄素进行10次平行测定,所得峰电流的相对标准偏差为2.0%。将该方法用于核黄素片剂和复合维生素B片剂的测定,结果令人满意。The electrochemical behaviors of riboflavin on bare gold electrode and gold electrodes modified with glutathion, with 3MPA, and with 2MBM are studied by cyclic voltammetry and differential pulse adsorption voltammetry. Experiment result shows that in BR buffer solution (pH 480), riboflavin exhibits a reversible wave at about -0.35 V(vs.SCE). The peak heights of differential pulse stripping voltammgram are linear to the riboflavin concentrations over the range of 3.0×10-7～2.3×10-4 mol/L, 1.05×10-6～2.0×10-4 mol/L, 2.1×10-6～2.08×10-4 mol/L and 105×10-6～2.0×10-4 mol/L. The correlation coefficients are 0.9932, 0.9909, 0.9857 and 0.9832, respectively. The detection limits are 2.1×10-4 mol/L, 5.2×10-4 mol/L, 8.6×10-4 mol/L and 5.2×10-4 mol/L, respectively. The relative standard deviation is 2.0% for 10 time measurements of 1.0×10-5 mol/L standard solution. This method is utilized for the determination of riboflavin in medicine, riboflavin tablets and compound vitamins tablets and satisfactory results are obtained. The recoveries are between 96%～104%.

关 键 词：核黄素 微分脉冲溶出伏安法 循环伏安法 金电极 分子自组装膜 电化学 

分 类 号：O629.4[理学—有机化学] O657.1[理学—化学]