La-phen-邻苯二甲酸混配化合物与DNA作用的共振光散射光谱及分析应用  

Long Range Assembly of Mixed-Complexes on the Molecular Surfaces of Nucleic Acid

作　　者：肖忠柏[1] 蔡朝霞[2] 李玲[2] 刘立明[2] 宋功武[2] 

机构地区：[1]江汉大学化学系,武汉430062 [2]湖北大学化学与材料科学学院,武汉430062

出　　处：《分析科学学报》2003年第4期317-320,共4页Journal of Analytical Science

摘　　要：本文研究了镧的混配物LaL1L2·3H2O(L1为邻苯二甲酸,L2为邻菲咯啉)与DNA作用的共振光散射光谱。加入DNA后,在pH6.5～7.5的范围内,LaL1L2·3H2O在DNA分子表面发生长距离自组装,在470nm处产生了增强的共振光散射峰,其发光强度与DNA的浓度成线性关系。该方法的线性范围为23～1870ng·mL-1,检出限(3σ)为23ng·mL-1,可用于测定纳克级的DNA。In this paper, resonance lightscattering characteristics of interaction between mixedcomplexes of LaL1L2Cl3·3H2O (L1∶C8H6O4;L2∶phen) with ctDNA were studied. When DNA was added, long range assembly of LaL1L2Cl3 on the molecular surfaces of nucleic acid occurred in the pH 6.5～7.5, resulting in an enhancement of resonance lightscattering (RLS) peak at 470 nm. The intensity of resonance lightscattering was found to be proportional to the concentration of DNA.

关 键 词：La-phen-邻苯二甲酸混配化合物 DNA 结构 共振光散射 光谱分析 长距自组装 镧系元素 

分 类 号：Q523[生物学—生物化学] Q503