微透析中空纤维膜接口的制作及其在二维毛细管电泳联用中的应用  

Fabrication of Microdialysis Hollow Fiber Membrane Interface and Its Application in Two-Dimensional Capillary Electrophoresis

作　　者：刘和春[1] 田燕[1] 杨春[1] 张维冰[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,辽宁大连116011

出　　处：《色谱》2003年第5期446-450,共5页Chinese Journal of Chromatography

基　　金：科技部"人类重大疾病蛋白组学研究"项目资助(No.973001CB510202).

摘　　要：提出了一种微透析中空纤维膜的制备方法,并用该微透析中空纤维膜作为接口构建了一套新型的二维毛细管电泳分离技术平台。中空纤维膜接口具有制作过程简易、传质效率高、柱间切换方便、分析速度快等优点,它是目前二维及多维电泳联用中一种较为新型、方便、理想的接口。以血红蛋白样品为例,通过实验验证了该接口在二维毛细管电泳联用系统中的可行性和分离效能。实验结果表明:整个血红蛋白样品二维分离分析的时间在1h内完成,二维分离系统的分辨率和总峰容量都比各自一维的高。A method for fabrication of microdialysis hollow fiber membrane is introduced. A novel interface using hollow fiber membrane was designed and fabricated, and an integrated twodimensional (2D) separation platform of proteome involving online coupling capillary isoelectric focusing (CIEF) with capillary zone electrophoresis (CZE) was constructed with the interface. The interface provided ease of fabrication, convenience of column　switching, free of columnswitching valve, and speed of mass delivering. Hemoglobin sample was selected to demonstrate the interface feasibility and resolving performance of the 2D CE system. The results show that the total analysis time of hemoglobin was less than 1 h, and the 2D CE separation system was found to have much higher resolving power and overall peak capacity in comparison with those obtained from either dimension alone.

关 键 词：微透析中空纤维膜 接口 制作 二维毛细管电泳 蛋白质 血红蛋白 

分 类 号：Q51[生物学—生物化学] Q503