Cloning and characterization of a mouse liver-specific gene mfrep-1,up-regulated in liver regeneration  

作　　者：JUNYAN HAOYING FEIGU JINHE YuLILI HuIMINLIU YONGHUAXU 

机构地区：[1]LaboratoryofMolecularandCellularOncology,InstituteofBiochemistryandCellBiology,ShanghaiIn-stitutesforBiolooicalSciences.ChineseAcademuofSciences.3120YueuanoRoad.Shanohai1200031.China [2]DepartmentofSurgery,HuashanHospital,FudanUniversitylShanghai1200031,China [3]DepartmentofPathology,ChangzhengHospital,SecondMilitaryMedicalUniversity,Shanghai1200003,China

出　　处：《Cell Research》2002年第5期353-361,共9页细胞研究（英文版）

基　　金：supported by research grants from the Special Funds for Major State Basic Research of China(Grant G1999053905)

摘　　要：Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-l/LFIRE-1), a liver-specific protein, is a member of fibrinogen superfamily that exerts various biological activities. However, the function of HFREP-l/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mouse fibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity to HFREP-l/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectively in mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA during regeneration after 70% partial hepatectomy (PHx) in mice, mfrep-1 mRNA increased in the regenerating liver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress the induction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNA continued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced expression of mfrep-1 maintained high until 72 h and then declined slowly to the basal level. Immunohistochemistry assessment confirmed the up-regulated expression of MFREP-1 protein in parenchymal cells during liver regeneration. These data suggested that MFREP-1 might play an important role in liver regeneration and be involved in the regulation of cell growth.

关 键 词：mfrep-1 in silico cloning liver regeneration liver-specific expression. 

分 类 号：R333.4[医药卫生—人体生理学] Q254[医药卫生—基础医学]