Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping  

作　　者：BANG LIU, GENG LIN JIN, SHU HONG ZHAO, MEI YU, TONG AN XIONG, ZHONG ZHEN PENG, Km LILaboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China 

出　　处：《Cell Research》2002年第5期401-406,共6页细胞研究（英文版）

基　　金：supported by the Key Project of National Basic Research and Developmental Plan(G2000016103)of China;the National Outstanding Youth Science Foundation(39925027);National Natural Science Foundation of China(39570519);the International Foundation for Science(IFS,B/2425-2F).

摘　　要：Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment com-bined with high chloroform Carnory’s fixative solution from cells of the testes of domestic pigs. Comparison in the division index and length of pachytene bivalents with metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87-5.98) times longer than those of the latter. Comparative studies on chromomere maps of bivalents and mitotic chromosomal G-bands were conducted by using the chromo-some 12 as a example. Sex vesicle and various shapes of synaptic sex chromosomes have been observed.Two-color PRimed IN Situ (PRINS) labeling has been conducted successfully on pachytene bivalents of pigs.

关 键 词：pachytene bivalents sex chromosome synapsis two-color PRINS microsatelHtes pigs. 

分 类 号：S828.1[农业科学—畜牧学]