基于硫化镉纳米团簇标记DNA电化学传感的研究  

Cadmium Sulfide Nanocluster-based Electrochemical Sensing of DNA Hybridization

作　　者：祝宁宁[1] 张爱平[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系

出　　处：《化学学报》2003年第10期1682-1685,共4页Acta Chimica Sinica

基　　金：国家自然科学基金(No .2 9972 0 0 8)资助项目

摘　　要：合成了表面具有自由羧基的硫化镉 (CdS)纳米团簇 ,以乙基 (3 二甲基丙基 )碳二亚胺盐酸盐 (EDAC)为偶联活化剂 ,将其标记于人工合成的 5′端氨基修饰的寡聚核苷酸 (ODN)片段上 ,制备成CdS纳米团簇标记DNA探针 ,该寡聚核苷酸片段与大肠杆菌肠毒素基因相关 .在一定的条件下 ,使其与固定在玻碳电极表面的待测DNA序列进行杂交反应 ,利用阳极溶出示差脉冲伏安法 (ASDPV)间接测定Cd(II)的量 ,实现对互补、非互补DNA片段的识别和电化学检测 。Synthesis of cadmium sulfide ( CdS) nanoclusters and its application in an electrochemical DNA hybridization detection were described. The nanoclusters were successfully labeled to a 5'-amino group capped oligonucleotides probe that is related to the colitoxin gene. The DNA genetic sensing assay relies on the target oligonucleotides on the glassy carbon electrode (GCE) hybridized with the CdS nanocluster-oligonucleotides DNA probe. Hybridization events between the probe and target were monitored by the release of the CdS anchored on the hybrids by oxidative dissolution and the indirect determination of the solubilized Cd2+ ions by sensitive anodic stripping voltammetry (ASV). The detection limit is 10 pmol/L of the complementary target oligonucleotides.

关 键 词：硫化镉纳米团簇 标记 DNA探针 电化学传感 杂交反应 

分 类 号：Q64[生物学—生物物理学]