鞘氨醇激酶基因在3T3-L1脂肪细胞诱导分化中的表达水平  

Expression of sphigosine kinase gene in 3T3-L1 preadipocyte differentiation

作　　者：潘晓勤[1] 郭锡熔[1] 丁胜利[1] 龚海霞[1] 倪毓辉[1] 费莉[1] 郭梅[1] 陈荣华[1] 

机构地区：[1]南京医科大学儿科医学研究所,南京210029

出　　处：《实用儿科临床杂志》2003年第10期808-810,T002,共4页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金 (项目编号 :3 9870 3 62 );江苏省教委自然科学基金(项目编号 :980 0 0 2 )

摘　　要：目的　探讨鞘氨醇激酶基因 (SPHK)在 3T3 L1脂肪细胞诱导分化中表达水平的变化。方法　采用细胞培养和RT PCR技术检测细胞分化不同阶段脂肪细胞中SPHK基因表达水平。结果　 1.SPHK基因在 3T3 L1脂肪前体细胞诱导分化初期表达呈明显上调趋势 ;2 .随着脂肪前体细胞分化成熟 ,该基因表达水平明显低于诱导分化初期的基因表达水平。结论　SPHK基因可能参与脂肪细胞分化的调控过程 。Objective To investigate the changes of sphigosine kinase gene expression during 3T3-L1 preadipocyte differentiation. Methods 3T3-L1 preadipocyte was differentiated into the adipocyte. Total RNA from the preadipocytes and adipocytes were extracted and the levels of sphigosine kinase gene mRNA expression were evaluated by RT-PCR.Results In early period of preadipocyte differentiation, the level of sphigosine kinase gene mRNA expression was significantly up-regulated. However, with adipocytes differentiating matured, the level of sphigosine kinase gene mRNA expression was significantly down-regulated, which was significantly lower than that in early period of preadipocyte differentiation. Conclusion Sphigosine kinase gene may be involved in adipocyte differentiation and adipogenesis.

关 键 词：鞘氨醇激酶基因 3T3-L1脂肪细胞 细胞分化 表达 肥胖 

分 类 号：R346[医药卫生—基础医学]