N^+离子注入对大豆种子活力及其幼苗的抗氧化酶活性影响  

Effects of N^+ Ion Implantation on Seed Vigor of Soybean and Some Antioxidase Activity in Soybean Seedling

作　　者：郭金华[1] 谢传晓[1] 徐剑 胡朝柱 余增亮[1] 

机构地区：[1]中科院离子束生物工程学重点实验室等离子体物理研究所,中国安徽合肥230031 [2]安徽丰乐种业农业科学技术学院,中国安徽合肥230031

出　　处：《激光生物学报》2003年第5期368-372,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金(19890300);"十五"国家科技攻关项目(2001BA302B)

摘　　要：本文研究了用25keVN+注入丰豆103的种子后,N+离子对其种子的活力及子叶伸展后48小时和96小时的幼苗内蛋白质含量、谷胱甘肽巯基转移酶(GSH-Ts)、谷胱甘肽过氧化酶(GSH-Px)、抗坏血酸过氧化物酶(ASA-POD)活性的影响。结果表明:当N+注量在2.6×1016N+/cm2～5.2×1016N+/cm2时,种子的发芽率、发芽指数、活力指数都明显提高;幼苗的可溶性蛋白含量高于对照。在6.5×1016N+/cm2～10.4×1016N+/cm2注量时,幼苗的可溶性蛋白含量低于对照,96小时幼苗可溶性蛋白的含量高于48小时,说明辐射引起的损伤可随生长时间的增加而有所恢复。高注量可引起幼苗内一些抗氧化酶活性的升高,且随注量的增加酶的活性升高也越明显,96小时幼苗的GSH-Px和ASA-POD活性高于48小时幼苗,GSH-Ts活性略有下降。而低注量(1.3×1016N+/cm2～5.2×1016N+/cm2)的上述酶指标升幅不大。说明经N+离子处理后可通过诱导这些抗氧化酶活性的升高起到减轻伤害的作用。Soybean seeds of Fengdou103 were implanted by 25keV N+ ion. Cotyledon samples(24h, 96h) were collected randomly during seedling stage from primary leavies for testing. The effects of N+ ion implantation (0 N+/cm2～10.4×1016N+/cm2) on the seed vigor content of soluble protein, activities of some glutathione transferase(GSHTs), glutathione peroxidase (GSHPx) and antioxidases including ascorbic acid peroxidase(ASAPOD) were investigated in the soybean seedlings. The results show that the germination rate, germination index and vigor index of soybean seed increase, and content of soluble protein in the soybean seedlings was higher than that in control when dose ranges from 2.6×1016N+/cm2 to 5.2×1016N+/cm2. The content of soluble protein on 96h was higher than that at 48h. It means that damage of the soybean seedlings induced by N+ion beams was repaired during the culture process.N+ ion implantation can increase activity of GSHTs,GSHPx and ASAPOD of the soybean seedlings .The activity of ASAPOD and GSHPx increased during the culture process and the activity of GSHTs decreased. It means that soybean seedlings can increase the activity of antioxidases to resist the damage induced by high N+ ion implantation. 

关 键 词：氮离子注入 抗氧化酶 大豆幼苗 种子活力 

分 类 号：Q691.5[生物学—生物物理学] S565.1[农业科学—作物学]