人发角蛋白修复大鼠脊髓横断损伤的实验研究(英文)  

EXPERIMENT STUDY OF HUMAN HAIR KERATIN TRANSPLANTED TO ADULT RATS AFTER SPINAL CORD CROSS - INJURY

作　　者：倪江东[1] 宋德业[1] 谢宏明[1] 李贺君[1] 谭进[1] 

机构地区：[1]中南大学湘雅二医院骨科,长沙410011

出　　处：《中国现代医学杂志》2003年第19期27-29,共3页China Journal of Modern Medicine

摘　　要：目的:研究人发角蛋白(HHK)在大鼠脊髓横断损伤(SCCI)修复中的作用。方法:选用18只SD大鼠,建立正常对照组、SCCI组,SCCI后植入HHK组。于术后1W,3W,6W对大鼠的运动,感觉进行评估;并在横断部位及近,远断3个断面分别取材,用HE,铀铅双染,在光镜及电子显微镜下观察其组织学结构。结果:大鼠脊髓横断损伤后植入HHK,运动,感觉逐渐好转,术后6W基本恢复正常;而未植入HHK大鼠无明显变化。光镜观察:植入HHK组白质有明显的神经纤维沿着HHK再生,部分区域有坏死,无明显的空洞形成;灰质有部分异常神经元,大部分形态正常。未植入HHK组断面有神经呈编织状再生,未见纤维超过离断面,有大面积坏死和空洞形成;灰质神经元大量坏死,形态异常。结论:HHK对横断脊髓的功能及形态恢复有促进作用,本实验为HHK应用于临床奠定基础。Objective:To study the function of human hair keratin [HHK] in repairing acute spinal cord cross -injury (SCCI) of SD adult rats. Methods: 18 adult female SD rats were selected and devided into three groups (control group, HHK group, SCCI group). Movement and sensation of SD adult rats were evaluated.The samples from spinal cord at injuryed or insertion site,9 and 11 precessus spinosus were observed with optical microscope and electronic microscope in 1, 3, 6 weeks after operation. Results:Campared with SCCI group, HHK group recovered better in sensation, motion and histological changes.Conclusions:HHK can repair spinal cord injury and promote the function recovery. HHK can boost the regeneration of nervous cord and reduce necrosis of nerve cells. This study is the base of clinical practices in treating spinal cord injury with HHK.

关 键 词：人发角蛋白 脊髓横断损伤 大鼠 

分 类 号：R681.5[医药卫生—骨科学]