猪组织中乳酸脱氢酶同工酶的分离与测定  

The Separation and Determination of Lactate Dehydrogenase Isozyme in Tissue of Pig

作　　者：詹新贵 金宏[2] 刘艳卉[2] 谭超[2] 傅童生[2] 

机构地区：[1]长沙市五里牌肉类联合加工厂,湖南长沙410001 [2]湖南农业大学动物科学技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2003年第5期426-427,共2页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省科技厅资助项目 ( 99JZY2 0 17)

摘　　要：为了探讨猪各组织器官中乳酸脱氢酶同工酶酶谱特征 ,采用聚丙烯酰胺凝胶电泳法比较分析了 12头三元杂交猪的心、肝、脾、肺等组织中乳酸脱氢酶同工酶酶谱的分布特征 .结果表明 ,肺、脾两组织中乳酸脱氢酶有 5条酶带 ,分别为 L DH1 ,L DH2 ,L DH3,L DH4 ,L DH5,其中 L DH3最宽 ,着色最明显 ;肝组织中只有 4条酶带 ,分别为L DH1 ,L DH2 ,L DH3,L DH5;心肌组织中只含 3条酶带 ,分别为 L DH1 ,L DH2 ,L DH3.pigs are used to analyze and investigate the isozyme spectra of lactate dehydrogenase (LDH) in tissue by means of polyacrylamide gel electrophoresis. The results are as follows: There are five bands in total on the LDH isozyme spectra in the lungs and spleen ,with LDH 3 the widest four bands of LDH in the liver(LDH 1,LDH 2,LDH 3 and LDH 5)and three bands of LDH in the heart (LDH 1,LDH 2 and LDH 3).

关 键 词：猪 组织 乳酸脱氢酶 同工酶 

分 类 号：S828.1[农业科学—畜牧学]