神经激肽-2受体的过度表达与慢性胰腺炎的疼痛相关(英文)  

Overexpression of neurokinin-2 receptor is related to pain in chronic pancreatitis

作　　者：石欣[1] 高乃荣[1] 霍明东[1] 杨永久[1] 胡浩霖[1] 郭庆明[2] Helmut Friess 

机构地区：[1]东南大学附属中大医院普外科,江苏南京210009 [2]东南大学附属中大医院病理科,江苏南京210009 [3]德国海德堡大学医院普外科

出　　处：《中国病理生理杂志》2003年第10期1369-1374,T005,共7页Chinese Journal of Pathophysiology

基　　金：TheprojectsponsoredbytheScientificRe searchFoundationfortheReturnedOverseasChineseScholars;StatePersonnelMinistry (No.76 90 0 0 4 0 2 7)

摘　　要：目的 :了解神经激肽 - 2受体 (NK - 2R)在正常胰腺和慢性胰腺炎组织中的表达 ,探讨其表达与慢性胰腺炎疼痛发生的关系。方法 :2 5个慢性胰腺炎标本取自慢性胰腺炎手术 ,男性 18例 ,女性 7例 ,正常胰腺组织取自 2 0个器官捐献者 ,男性 11例 ,女性 9例。应用实时定量逆转录聚合酶链反应技术 ,检测正常胰腺和慢性胰腺炎组织NK - 2R的mRNA水平 ,应用Westernblot技术检测NK - 2R的蛋白水平 ,应用免疫组织化学方法进行NK - 2R的组织学定位。将NK - 2RmRNA水平与疼痛的程度、发作频率和持续时间进行分析 ,寻找其中的相关性。结果 :与正常胰腺相比 ,慢性胰腺炎组织中NK - 2RmRNA和蛋白都过度表达 ,NK - 2RmRNA水平与疼痛的程度 (r =0 .5 9,P <0 0 1)、发作频率 (r=0 .5 1,P <0 0 5 )和持续时间 (r=0 5 3,P <0 0 5 )明显相关。免疫组化检查显示 ,NK - 2R的表达主要位于胰腺腺泡、胰腺导管、神经纤维和炎性细胞。结论 :慢性胰腺炎组织中NK - 2R的mRNA和蛋白表达水平都明显上调 ,扰乱了神经激肽的作用环节 ;NK - 2R的过度表达与疼痛发生有关。AIM: To investigate the expression of neurok inin-2 receptor (NK-2R) in normal pancreas and chronic pancreatitis (CP) tissues. The relation of expres sion of NK-2R with pain in CP was also evaluated. METHODS: CP tis sues were ob tained from 18 men and 7 women undergoing pancreatic head resection as a result of CP. Normal human pancreatic tissues were obtained from 20 patients (11 males/ 9 females). Real-time quantitative reverse transcription polymerase chain reacti on was used to determine the mRNA expression of NK-2R, Western blot analysis was used to determine its protein expression level, and immunohistochemistry was us ed to localize expression site of NK-2R protein. NK-2R mRNA level and pain were also analysed whether correlation exists. RESULTS: NK-2R mRNA and p rotein level were enhanced expressed in CP tissue samples compared with normal pancreas. Ove rexpression of NK-2R was related to the intensity ( r=0 59, P <0 01), freque nc y ( r=0 51, P <0 05) and duration ( r=0 53, P <0 05) of the pain in CP p atie nts. Immunohistochemistry revealed that relatively stronger NK-2R immunoreactivi ty was observed in acinar and ductal cells as well as in fibroblast-like cells a nd in the bl ood vessels in CP tissues. CONCLUSIONS: NK-2R is overexpressed in CP , which may exacerbate the pathophysiological change in CP, and be involved in t he pathogenesis of pain in this disease.

关 键 词：胰腺炎 受体 神经激肽2 疼痛 基因过度表达 

分 类 号：R657.51[医药卫生—外科学]