降香黄檀根际固氮菌分离鉴定及菌群活性研究  

Screening and Identification of Azotobacter in Rhizosphere of Dalbergia odorifera and Research on Activities of Microflora

作　　者：徐睿[1] 刘君昂[1] 罗娜[1] 邓雷[1] 刘慧娟[1] 周国英[1] 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙410004

出　　处：《土壤通报》2015年第5期1121-1126,共6页Chinese Journal of Soil Science

基　　金：国家林业公益性行业科研专项(201304402)资助

摘　　要：利用Ashby培养基从不同林龄的降香黄檀根际土壤中分离高效固氮菌,为专用生物复合肥的研制提供理论基础。固氮酶活在75.30~432.76 nmol h-1 ml-1之间的菌株有21株,其中菌株JT-N305的固氮酶活最高。其中15株菌株具有分泌IAA的能力,其分泌量在9.76~41.21μg ml-1。结合形态学、生理生化特性及16S r DNA序列分析,初步鉴定JT-N301属于Burkholderia anthina、JT-N304属于Enterobacter ludwigii、JT-N305属于Burkholderia seminalis。菌群的研究表明:3株菌株混菌培养,其固氮酶活高达498.66 nmol h-1 ml-1;相对于其它氮源而言,菌群更易于吸收铵盐;而菌群的最佳碳源为葡萄糖。In order to provide th eoretical basis for the development of the special biological compound fertilizer, the high- efficiency Azotobacter were screened from different ages of Dalbergia Odorifera rhizosphere soil by Ashby medium. Of the isolate strains, 21 strains possessed higher nitrogen- fixing capacity, with the range of nitrogen live from 75.30 nmol h- 1m L- 1to 432.76 nmol h- 1m L- 1, among which strain JT- N305 showed the highest nitrogen- fixing capacity. 15 of the 21 strains had the ability to produce IAA, with the range of IAA- producing from 9.76 μg m L- 1to41.21 μg m L- 1. Based on the morphology, physiological and biochemical property and 16 S r DNA sequence analysis,JT- N301, JT- N304 and JT- N305 were preliminarily identified as Burkholderia anthina, Enterobacter ludwigii and Burkholderia seminalis, respectively. The research of 3 strains mixed culture showed that the nitrogenase activity was up to 498.66 nmol h- 1 m L- 1. Compared with other nitrogen sources, ammonium salt was more likely to be absorbed by the mixed microflora. The optimum carbon source was glucose.

关 键 词：降香黄檀 根际固氮菌 固氮酶活 IAA 

分 类 号：S714.3[农业科学—林学]