非洲爪蟾卵非细胞体系在药物筛选方面应用初探  

The preliminary application of Xenopus leavis eggs cell-free system in drug screening

作　　者：田辉凯[1] 姚小皓[1] 张红梅[1] 李学军[1] 

机构地区：[1]北京大学基础医学院药理学系,北京100083

出　　处：《中国药理学与毒理学杂志》2003年第4期251-256,共6页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的　为寻找具有分子水平、特异、快速、可用来筛选具有调节凋亡作用药物的方法 ,非洲爪蟾卵非细胞体系 (XS 2 0 0 )作为一种药物的筛选平台 ,研究其作为筛选药物方法的可能性。对体系内凋亡过程的影响进行了初步的研究。方法　药物 (激素 :雌激素及其部分拮抗剂 ,中药提取物 :灵芝三萜和多糖 ,小分子药物 :阿司匹林、水杨酸钠和乙酰唑胺 ,维生素C及重组蛋白rhTFAR19)预先与XS 2 0 0共孵育 ,然后加入阈剂量 (0 .0 6 2 5 μmol·L- 1)的细胞色素C和小鼠肝细胞核 ,提取总DNA进行电泳鉴定。结果　rhTFAR19对XS 2 0 0凋亡过程有直接的促进作用 ,而其他药物则无明显作用。结论　XS 2 0 0可以成为一个良好的分子水平的药物筛选平台 ,特异筛选具有调节凋亡作用的药物。AIM To find a molecular, specific and efficient drug screening method for apoptosis, we studied the possibility of Xenopus leavis eggs cell free system (XS 200) and observed the process of apopotosis in system. METHODS We studied the influence of different kinds of drugs including hormone: estradiol and its partial antagonist anordiol; the extracts of Chinese herb: triterpenes and polysaccharides; small molecular drug: aspirin, sodium salicylate and a new compound ZXS 1; vitamin C and recombinant protein: rhTFAR19 on apoptosis in XS 200. XS 200 was pre incubated with drugs for 30 min and added with cytochrome C of threshold (0.0625 μmol·L -1 ) and mouse liver nuclei. Total DNA was prepared and identified by agarose gel electrophoresis. RESULTS Only rhTFAR19 could promote the apoptotic process in XS 200. CONCLUSION XS 200 could become a useful tool for drug screening.

关 键 词：无细胞系统 凋亡 药物筛选 

分 类 号：R965.1[医药卫生—药理学]