SEN病毒D亚型中国株流行病学及序列变异  

Epidemiology and molecular evolutionary analysis of Chinese SEN virus D subtype

作　　者：杜娟[1] 詹林盛[1] 李娅[2] 王海平[1] 孟庆华[3] 彭剑淳[1] 王全立[1] 

机构地区：[1]军事医学科学院野战输血研究所 [2]军事医学科学院附属医院 [3]北京市佑安医院肝病三科

出　　处：《中华微生物学和免疫学杂志》2003年第8期625-628,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　了解SEN病毒D亚型中国株的流行病学特点及其进化地位。方法　分析已知的基因序列设计引物 ,建立半套式PCR检测方法 ,对 5 8份献血者血清样本和 2 5份non A～E肝炎患者血清样本进行了SENV D亚型的检测 ,并对部分阳性血清的PCR产物进行克隆测序。结果　无偿献血者中SENV D亚型感染率为 4 5 % ,在non A～E肝炎患者中SENV D亚型感染率为 4 8% ,两者间差异无显著性 ;但在无偿献血者中SENV D亚型的阳性检出率大大高于国外报道。测序结果经BLASTP软件在GenBank中检索表明 ,各测序株均与已知的SENV D株序列有很高的同源性 ,并在此基础上用MegAlign软件进行进化树分析。结论　建立的半套式PCR检测方法适用于SEN病毒D亚型检测 ;SENV D亚型中国株之间以及与已发表的国外SENV D株序列之间有变异。与无偿献血者相比 ,non A～E肝炎患者血清SENV D株无进化上的显著倾向性。在测序的部分ORF1序列中存在一个核苷酸高变区。Objective To investigate the epidemiology and mo lecular evolution of Chinese SEN virus D. Methods Accordi ng to the published gene sequences, we designed primers from the upstream reserv ed region. Half nested-PCR methods for SENV-D were built, the 58 samples from the voluntary blood donors and 25 samples from the non-A～E hepatitis patients were detected by this method. Some PCR products were cloned and sequenced. Results The incidence of SENV-D was 45% in the voluntary bloo d donors and 48% in the non-A-E hepatitis patients. There was no significant d ifference between the two groups. The positive rate of SENV-D was higher than t hat reported abroad. The BLASTP search revealed these sequences with a high perc entage of homology with published SENV-D sequences. The unrooted phylogenetic t ree on this basis was created. Conclusion We successfully established half nested-PCR method for the detection of SENV-D. Based on this short fragment, the SENV-D strains coming from the non-A non-E hepatitis pat ients had no distinct evolutionary tendence compared with blood donors. There wa s a hypervariable region in this short fragment.

关 键 词：SEN病毒 D亚型 流行病学 序列变异 半套式聚合酶链反应 

分 类 号：R446.61[医药卫生—诊断学]