银杏叶提取物对豚鼠肠系膜下神经节细胞生物电的影响  

Bioelectrical Effect of Ginkgo Biloba Extract on Neurons ofGuinea Pig Inferior Mesenteric Ganglion in vitro

作　　者：柯道平[1] 孔德虎[1] 王刚[1] 张玲[1] 黄振信[1] 祝延[1] 

机构地区：[1]安徽医科大学生理学教研室,安徽合肥230032

出　　处：《安徽中医学院学报》2003年第5期52-55,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：安徽省教育厅科研基金项目 (2 0 0 2KJ14 7)

摘　　要：目的 :通过观察银杏叶提取物 (EGb)对交感神经节细胞生物电的影响 ,为进一步探讨EGb调节肠系膜下神经节 (IMG)支配的包括远端结肠在内的内脏功能活动的作用机制提供依据。方法 :采用细胞内生物电记录技术记录体外IMG电位。结果 :在敏感细胞 ,EGb76 1(35～ 35 0mg/L)可使 71.0 %的肠系膜下神经节细胞产生膜去极化反应 ,且在一定范围内呈现剂量效应关系 ;19.4 %的细胞超极化 ;9.7%的细胞出现先超极化后去极化的双相反应。去极化反应的细胞其膜电阻有降低、增大和不变等多种表现。此去极化反应可被低钙 高镁克氏液部分阻断 ,但不为胆碱和肾上腺素受体阻断剂所阻断。还发现EGb76 1可抑制外源性 5 HT引起的去极化电位。结论 :EGb76 1对IMG细胞可能通过非胆碱能、非肾上腺素能通路产生易化作用 ;Objective:To investigate the bioelectrical effects of ginkgo biloba extract (EGb) on neurons of sympathic ganglions,and further reveal the mechanism of EGb in regulating colon function innervated by inferiormesenteric ganglion (IMG). Method:Intracellular recordings were performed from neurons of the isolated guinea pig IMG. Result:Superfusion of the IMG with EGb761 induced (35～350 mg/L) induced a dose dependent membrane depolarization in the majority (71.0%) neurons; 19.4% cells were evoked a hyperpolarization and 9.7% neurons a biphasic response with an initial depolarization and followed hyperpolarization. The EGb761 induced depolarization associated with a variety of input membrane resistance (Rm) changes including marked decrease, increase and no apparent change. The EGb761 induced depolarization was partially changed in low Ca 2+ and high Mg 2+ solution, but not changed appreciably by cholinergic receptor and adrenergic receptor antagonists. EGb761 also attenuated the depolarization induced by exogenously applied 5 HT. Conclusion: EGb761 can produce an electrophysiological action on IMG neurons by non cholinergic and non adrenergic systems,and the mechanism may associate with influence on the release of other neurotransmitters.

关 键 词：银杏叶提取物 豚鼠 肠系膜 神经节 细胞生物电 

分 类 号：R285.5[医药卫生—中药学]