β-细辛醚对小鼠脑组织基因表达谱的影响  

Effects of β-asaron on Gene Expression in Mouse Brain

作　　者：方永奇[1] 李翎[1] 吴启端[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《中药材》2003年第9期650-652,共3页Journal of Chinese Medicinal Materials

摘　　要：目的 :应用基因芯片技术 ,观察 β 细辛醚对BALB/c小鼠脑组织基因表达谱的影响。方法 :将 β 细辛醚用药组与空白对照组小鼠脑组织的mRNA分别用cy5、cy3荧光标记 ,混合后与 4 0 0 0条基因杂交于MGEC - 4 0S小鼠表达谱芯片上 ,重复制作二张片 ,Genepix 4 0 0B扫描仪扫描杂交信号荧光强度 ,GenepixPro3 0图象处理软件分析扫描结果。结果 :在 4 0 0 0条基因中 ,二张片中两组都出现差异表达且比值大于 1 9或小于 0 5 9的基因共 15条。β 细辛醚组有 4条基因表达上调 ,11条基因表达下调。表达上调的 4条基因主要与离子通道、细胞的跨膜物质变换、钙依赖性蛋白激酶调节、减少细胞凋亡等功能有关。而表达下调的 11条基因主要与脑内兴奋性氨基酸的代谢、T淋巴细胞的趋化作用、细胞基因表达调控、药物代谢等功能有关。结论 :β 细辛醚对脑组织多个靶基因有作用。Objective: To observe effects of β-asaron on gene expression in BALB/c mouse brain by gene chip. Methods: The mRNA of mouse brain samples of experiment group and control group was markered with cy5, cy3 fluorescence separately, and hybridized with 4000 genes on MGEC-40S gene chip after mix. Fluorescence intensity was scanned with Genepix 400 B scanner and analysed with Genepix Pro3.0 soft. Results: Among 4000 genes, there are 15 genes with difference expression (the ratio>1.9 or <0.59) in two groups. In β-asaron group, 4 genes expression raised relating to ion channels, substance transform through membrane, calcium-dependent protein kinase regulation, apoptosis, 11 genes expression declined relating to excitatory neurotransmitter metabolism in the CNS, T lymphocyte tend, genes expression regulation and drug metabolism. Conclusion:β-asaron has effects on multi-target genes in mouse brain.

关 键 词：Β-细辛醚 小鼠 脑组织 基因表达谱 石菖蒲 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]