受细菌诱导的植物启动子的克隆及其在转基因烟草中的活性  

Cloning of bacteria-inducible plant promoters and their activation in transgenic tobacco

作　　者：彭建令[1] 董宏平[1] 包志龙[1] 董汉松[1] 王金生[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2003年第3期36-40,共5页Journal of Nanjing Agricultural University

基　　金：国家 973计划 (G2 0 0 0 0 162 0 1);国家自然科学基金 (3 0 0 80 0 11);江苏省农业高技术项目 (BG2 0 0 13 0 7)

摘　　要：从烟草中克隆了受病原物诱导的植物启动子 (pathogen inducibleplantpromoters,PPPs)PPP1、PPP2、PPP3,其长度分别为 130 8,1170 ,2 2 0bp ,序列分析表明它们均含有受细菌诱导的反应元件 (Bac)。资料检索表明 ,PPP3可能是从植物分离的、受细菌诱导的最短的启动子。构建了包括每个PPP启动子和报道基因uidA (编码 β 葡萄糖醛酸酶 ,GUS)的转化单元 ,用它们以及包括来自椰菜花叶病毒 35S启动子和uidA单元 ,在相同条件下分别转化烟草 ,获得了分别含PPP和 35S启动子的 4类转基因烟草品系 ;分子检测表明 ,转化单元在转基因烟草染色体上能稳定整合。根据对转基因烟草GUS组织化学测定 。Pathogen inducible plant promoters(PPPs) could be used to define signaling pathways and engineer crops for disease resistance.PPP1,PPP2 and PPP3,with size of 1 308,1 170 and 220 bp,respectively,were cloned from tobacco.Sequence analyses revealed a bacteria inducible element in the three promoters.Four species of transgenic tobacco were generated following transformation with constructs containing individuals of the PPP and cauliflower mosaic virus 35S promoters,fused with the β glucuronidase(GUS) gene.According to the PCR analysis,the PPPs were stably integrated into the tobacco genome.Histochemical staining of transgenic tobacco leaves for GUS activities suggests that the PPPs were induced by Ralstonia solanacearum.

关 键 词：植物 克隆 转基因烟草 反应元件 病原物诱导性启动子 细菌诱导 诱导抗病性 

分 类 号：S432.23[农业科学—植物病理学]