增液汤对急性伤阴动物模型的细胞保护作用及其机理探讨  

A Research into Cell Protection and Mechanism of Zengyetang on Acute Impairment of Yin Amimal Models

作　　者：仝小林[1] 王君[1] 李宁[1] 王红[1] 曹丽英[2,3] 叶智文 

机构地区：[1]中日友好医院 [2]北京科学出版社 [3]北京100700 [4]中日友好医学临床研究所 [5]北京100029

出　　处：《中国中医基础医学杂志》2003年第8期45-47,共3页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：Acute Yin impairment is a frequently occurring clinic syndrome. We suggest the process of acute Yin impairment be divided into two stages, namely early impairment of lung and stomach fluid and later Yin impairment of liver and kidney. This work aims to research into the cell protecting mechanism of Zengyetang, from the angle of protecting cells, change of inner and outer fluid ion of cells, change of enzyme of cell membrane, free radical impairment, appoptosis and gene expression. The study, taking inner and outer fluid ion of cells as objective indicators, also researches into dynamic change of inner and outer fluid of cells in the different stages of Yin impairment development on the animal models of yin impairment by fever and poison. Zengyetang is one of the typical Yin nourishing prescriptions. Zengyetang is made into injection in accordance with the rate of the original prescription. This work researches into the affects of Zengyetang on the four types of acute Yin impairment animal models, including Yin impairment model by pathogenic heat, Yin impairment model by summer heat and sweat, Yin impairment model by high infiltration Yin impairment and Yin impairment by drugs and toxin. Main experiment results show:1. in early stage, pathogenic heat Yin impairment impairs outer liquid of cells; 2. Zengyetang has the function of adjusting disorder of inner and outer fluid of red cells; 3. Zengyetang has the function of protecting enzyme activity of cell membrane and maintaining normal operation of red cell ion; 4. Zengyetang can protect liver cells of rabbits through resisting free radical impairment and lessen thymocyte apoptosis of young rat resulted from glucocorticoid.Acute Yin impairment is a frequently occurring clinic syndrome. We suggest the process of acute Yin impairment be divided into two stages, namely early impairment of lung and stomach fluid and later Yin impairment of liver and kidney. This work aims to research into the cell protecting mechanism of Zengyetang, from the angle of protecting cells, change of inner and outer fluid ion of cells, change of enzyme of cell membrane, free radical impairment, appoptosis and gene expression. The study, taking inner and outer fluid ion of cells as objective indicators, also researches into dynamic change of inner and outer fluid of cells in the different stages of Yin impairment development on the animal models of yin impairment by fever and poison. Zengyetang is one of the typical Yin nourishing prescriptions. Zengyetang is made into injection in accordance with the rate of the original prescription. This work researches into the affects of Zengyetang on the four types of acute Yin impairment animal models, including Yin impairment model by pathogenic heat, Yin impairment model by summer heat and sweat, Yin impairment model by high infiltration Yin impairment and Yin impairment by drugs and toxin. Main experiment results show:1. in early stage, pathogenic heat Yin impairment impairs outer liquid of cells; 2. Zengyetang has the function of adjusting disorder of inner and outer fluid of red cells; 3. Zengyetang has the function of protecting enzyme activity of cell membrane and maintaining normal operation of red cell ion; 4. Zengyetang can protect liver cells of rabbits through resisting free radical impairment and lessen thymocyte apoptosis of young rat resulted from glucocorticoid.

关 键 词：增液汤 急性伤阴 动物模型 细胞保护 作用机理 阴津 

分 类 号：R256[医药卫生—中医内科学] R289.5[医药卫生—中医学]