免疫滴金技术检测肾综合征出血热特异性抗体的研究  

A study on detecting specific antibody of hemorrhagic fever with renal syndrome with colloidal gold-dot assay

作　　者：储峰 季青 严润民 王霞明 裴彬 

机构地区：[1]上海市南汇区南华医院,上海201300

出　　处：《上海预防医学》2003年第10期492-494,共3页Shanghai Journal of Preventive Medicine

基　　金：上海市科委科研基金资助 (97491 90 2 1 )

摘　　要：[目的 ]　为探索一种更为简便、快速、特异、灵敏的肾综合征出血热 (HFRS)抗体的检测方法。　 [方法 ]　45 8例HFRS病人血清同时采用免疫滴金法 (CGIDA)与酶联免疫吸附法 (ELISA)对比检测特异性免疫球蛋M抗体 (抗HFRS -IgM )、间接免疫荧光法 (IFAT)对比检测特异性免疫球蛋G抗体 (抗HFRS -IgG) ,并以 2 0例发热待查、48例病毒性肝炎血清作对照。对 15 2例肾综合征出血热病人作肝功能检查。　 [结果 ]　 45 8例HFRS病人血清 ,以CGIDA法检测抗HFRS -IgM ,阳性 3 5 8例 ,与ELISA法作结果评价时 ,CGIDA灵敏度 79.9% ,特异度 10 0 % ;以CGIDA法检测抗HFRS-IgG ,阳性 3 86例 ,与IFAT法作结果评价时 ,CGIDA灵敏度 91.5 % ,特异度 10 0 %。 15 2例肝功能检查发现 86例ALT升高 ,占 5 6.6% ,胆红素升高者 3 3例 ,占 2 8.3 %。　 [结论 ]　CGIDA法检测HFRS特异性抗体分别与ELISA法及IFAT法对照 ,均有简便、快速、特异、灵敏之优点 ,检测抗HFRS -IgM ,CGIDA法敏感性差于ELISA法 ,但特异性较高 ;检测抗HFRS -IgG ,CGIDA法的灵敏度高于IFAT法。肝功能检查表明 ,肾综合征出血热并发肝损害发生率较高 ,临床表现轻 。Objective To explore a simple, speedy, specific and sensitive method to detect specific antibody of hemorrhagic fever with renal syndrome(HFRS). Methods The serum of 458 patients with HFRS were tested with colloidal gold immuno-dot assay(CGIDA) for SIgM and SIgG antibody. The resulfs were compared with those from enzyme linked immunosorbent assay(ELISA) or indirect fluorescent antibody test(IFAT) and the control group(20 cases with fever of unknown and 48 hepatitis of viral cases). 152 cases were tested for liver function. Results When compared with ELISA, the rate of specificity of detection of SIgM of CGIDA was 100% and its sensitivity was 79.9%. When compared with IFAT, detection of SIgG of CGIDA was also 100%, sensitivity was 91.5%. 86 of the 152(56.6%) cases tested for liver function had elevated ALT and 33 of 152(28.3%)with elevated serum billirubin. Conclusion CGIDA was simple and speedy, in detecting the SIgM or SIgG antibody in serum of patients with HFRS, and was more specific and sensitive than ELISA or IFAT. Though the sensitivity of CGIDA was lower than that of ELISA, CGIDA had no false positive reaction; the sensitivity of CGIDA was higher than that of IFAT in detecting anti-HFRS IgG. The result of liver function test showed that the liver injuries were common in HFRS but clinical features were light, and mostly recovered at the diuresis stage.

关 键 词：免疫滴金技术 检测 肾综合征出血热 特异性抗体 研究 酶联免疫吸附法 

分 类 号：R446.6[医药卫生—诊断学]