丹参对兔急性胰腺炎早期中性粒细胞与内皮细胞黏附抑制作用的实验研究  

Experiment study of salvia miltiorrhiza on inhibiting polymorphonuclear leukocyte and endothelial cells adhesion in acute pancreatitis rabbits

作　　者：沙建平[1] 祝彼得[1] 徐艳[1] 陈炫[2] 赵艳[1] 刘啸[1] 芦殿荣[1] 刘舟[1] 

机构地区：[1]成都中医药大学,四川成都610072 [2]四川大学华西医学院,四川成都610041

出　　处：《中国中西医结合急救杂志》2003年第5期279-281,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目 (3 0 2 71661)

摘　　要：目的 :探讨中性粒细胞 (PMN)与内皮细胞 (EC)黏附在急性胰腺炎 (AP)早期发病中的机制及丹参对AP的治疗作用。方法 :采用兔 AP模型 ,2 4只日本大耳兔随机分为生理盐水组和丹参治疗组。分别于术前、术后即刻及 2、4、8、12、16和 2 4 h取血分离获得 PMN,与体外培养的兔血管 EC作用 ,测定 PMN EC黏附率及PMN表面黏附分子 CD11a/ CD18和 CD11b/ CD18值。结果 :术后即刻两组黏附分子 CD11a/ CD18、CD11b/CD18及 PMN EC黏附率均升高 ,生理盐水组术后 4 h达到高峰 ;丹参治疗组术后 2、4、8、12、16和 2 4 h的CD11a/ CD18、CD11b/ CD18显著低于生理盐水组。结论 :丹参能降低黏附分子 CD11a/ CD18、CD11b/ CD18表达 ,抑制 PMN EC黏附 ,改善微循环及减轻 PMN EC黏附所致的组织损伤 ,有助于 AP的早期治疗。Objective: To study the mechanism of polymorphonuclear leukocyte(PMN) and endothelial cell(EC) adhesion in the early period of acute pancreatitis(AP) and the effect of salvia miltiorrhiza on AP. Methods : Rabbit AP model was established. Twentyfour Japan rabbits were divided randomly into normal saline group which was treated with normal saline and the salvia miltiorrhiza treatment group which was treated with saliva miltiorrhiza. Blood was obtained at prooperation,postoperation instantly, 2 hours, 4 hours , 8 hours, 12 hours, 16 hours and 24 hours after operation. PMN was purred out of the blood, and interacted with rabbit's vascular EC which was cultured out of the body , the adhesion ratio of PMNEC and CD11a/CD18, CD11b/CD18 expression was measured. Results: After operation, both CD11a/CD18, CD11b/CD18 experssion and the PMNEC adhesion ratio were increased immediately, the normal saline group reached the peak at 4 hours after the operation, the salvia miltiorrhiza treatment group was dramatically lower compared with those of the normal saline group at 2 hours, 4 hours, 8 hours, 12 hours, 16 hours and 24 hours after operation. Conclusion: Salvia miltiorrhiza can lower the CD11a/CD18,CD11b/CD18 expression, inhibit PMNEC adhesion, improve the microcirculation, ease the tissue damage produced by PMNEC adhesion and help the AP treatment in the early period.

关 键 词：丹参 急性胰腺炎 中性粒细胞 内皮细胞 动物模型 细胞培养 黏附率 微循环 

分 类 号：R285.5[医药卫生—中药学]