羚蝎胶囊对局灶性脑缺血-再灌注大鼠模型循环内皮细胞的影响  

Effect of Lingxie capsule(羚蝎胶囊) on circulating endothelial cells of rats with focal cerebral ischemia/ reperfusion injury

作　　者：朱冬胜[1] 麻志恒[2] 陈忆伟[1] 祝鋆[1] 王方[1] 卫洪昌[2] 徐敏华[1] 

机构地区：[1]上海市第二人民医院,上海200011 [2]上海中医药大学,上海200032

出　　处：《中国中西医结合急救杂志》2003年第5期296-298,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：上海市黄浦区科委资助课题 (2 0 0 2 HCG 15 )

摘　　要：目的 :观察羚蝎胶囊对脑缺血再灌注损伤模型大鼠循环内皮细胞 (CEC)的影响 ,探讨其治疗脑梗死的可能机制。方法 :36只 SD大鼠随机分为假手术组、模型组和羚蝎胶囊治疗组 ,用大脑中动脉栓塞 (MCAO)法复制大鼠局部脑缺血再灌注模型 ,观察各组大鼠神经功能缺损评分及血浆 CEC的变化。结果 :羚蝎胶囊组再灌注后 3h神经功能缺损评分〔(2 .30± 0 .34)分 )〕显著低于模型组同期〔(3.10± 0 .2 3)分〕;羚蝎胶囊组血浆CEC水平〔(5 .5 7± 0 .89)个 / 0 .9μl〕较模型组低〔(6 .38± 0 .91)个 / 0 .9μl,P<0 .0 5〕。结论 :羚蝎胶囊能降低局部脑缺血再灌注损伤模型大鼠的 CEC数量 ,这可能是该方治疗脑梗死的机制之一。Objective: To observe the effect of Lingxie capsule(羚蝎胶囊) on circulating endothelial cells (CEC) of rats with focal cerebral ischemia/reperfusion(I/R) injury, and to investigate the mechanism of Lingxie capsule in treatment of cerebral infarction. Methods: Thirtysix SD rats were randomly divided into three groups, the shamoperated group, the control group and the Lingxie capsule treatment group. Rat model of cerebral I/R injury was established by the middle cerebral artery occlusion. The neurofunction deficit score and CEC were observed in cerebral I/R model rats. Results: Significant difference was found between the control group and the Lingxie capsule treatment group in decrease of neurofunction deficit score 3 hours after operation 〔(2.30±0.34) scores in Lingxie capsule treatment group and (3.10±0.23) scores in model group, P <0.05〕 and the count of CEC 〔(5.57±0.89) cells/0.9 μl in Lingxie capsule treatment group and (6.38±0.91)cells/0.9 μl in model group, P <0.05〕. Conclusion: Lingxie capsule might play an important role in reducing CEC of rats with focal cerebral I/R injury, and it may be the mechanism of treating cerebral infarction .

关 键 词：羚蝎胶囊 局灶性脑缺血 再灌注 大鼠 内皮细胞 神经功能缺损 药理作用 

分 类 号：R285.5[医药卫生—中药学]