HPLC法测定人血浆中劳拉西泮的浓度  

Determination of lorazepam in blood plasma by HPLC with UV detection

作　　者：温预关[1] 陈伟家[1] 叶莉英[1] 陆欣乔[1] 

机构地区：[1]广州市脑科医院临床药理研究基地,广州510370

出　　处：《中国临床药学杂志》2003年第5期286-288,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的:建立测定人血浆中劳拉西泮浓度的HPLC法。方法:采用美国Dikma公司Diamonsil^(TM)C_(18)(250mm×4.6mm,5μm)为色谱柱,流动相为甲醇-水(75:25,V/V),流速为0.8mL·min^(-1),检测波长232mm,以乙酸乙酯为提取溶剂。结果:劳拉西泮高(20.00μg·mL^(-1))、中(6.00μg·mL^(-1))、低(0.60μg·mL^(-1))3个浓度的平均回收率分别为101.67％,98.17％,91.20％,日内、日间RSD均<5％(n=5);分析方法的定量测定下限为0.O05μg·mL^(-1)。线性范围为0.01～20.00μg·mL^(-1),回归方程为Y=2.16×10^(-2)X-3.33×10^(-3),r=0.9999(n=10)。结论:该方法灵敏、准确、简单、快速,可用于劳拉西泮临床血药浓度监测和药动学研究。AIM: To develop an HPLC method for quantitative determination of lorazepam in blood plasma. METHODS: Blood plasma samples were extracted with hexane to remove lipid and interfering substances; lorazepam was then extracted with ethyl acetate. The residues dissoved were analyzed with a RP-HPLC system (C18 column, 250 mmx 4.6 mm, 5um); Mobile phase, MeOH-H2O (75:25, V/V); UV detection, 232 nm. RESULTS: The average recoveries for lorazepam were 101.67% ,98.17% , 91.20% , respectively. The within-day and between-day relative standard deviations were smaller than 5% ( n=5) . The calibration curves had good linearity, r= 0.999 9 within a concentration range of 0.01 -20.00 ug mL-1. The limit of quantitation for lorazepam was 0.005 ug mL-1. CONCLUSION: The method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of lorazepam blood plasma and its phamacokinetic studies.

关 键 词：HPLC法 浓度测定 人血浆 劳拉西泮 血药浓度 高效液相色谱法 

分 类 号：R96[医药卫生—药理学]