生物活性短肽RGD在PET表面接枝方法的研究  

Research in Synthesis of Bioactive Peptide RGD and the Method for Its Grafting on PET Surface

作　　者：赵强[1] 万昌秀[2] 刘建伟[2] 邱凯[2] 

机构地区：[1]四川大学华西药学院,成都610041 [2]四川大学高分子材料系,成都610065

出　　处：《生物医学工程学杂志》2003年第3期384-387,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (5 9973 0 12 )

摘　　要：在高分子材料表面共价引入人工合成的精氨酰 -甘氨酰 -天冬氨酸三肽 (Arg- Gly- Asp peptides,RGD) ,以达到让内皮细胞与之特异结合并更加牢固的目的。实验用紫外辐照法将活性基团羧基 (- COOH)接枝到聚对苯二甲酸乙二醇酯 (Poly(ethylene terephtalate) ,PET)膜的表面 ,将液相合成的 RGD三肽耦合接枝到处理过的材料表面 ,光电子能谱对以羧基为活性基团的接肽反应结果进行分析 ,光学、电子显微镜观察内皮细胞生长情况以检测接枝短肽的生物活性。内皮细胞生长实验结果表明 ,成功接枝的 RGD序列对材料内皮细胞种植起到了促进作用。本实验成功运用紫外接枝与化学耦合 ,将生物活性短肽 RGD接枝到膜表面 ,探索了一种新的接枝生物活性短肽的方法。The aim of this experiment is to graft synthesizing Arg-Gly-Asp peptides (RGD) on the surface of polymer materials, combine endothelial cells with its special site, enhance the adhesion of endothelial cells on the surface, promote the blood compatibility of the biomaterials. Carboxy group (-COOH) was grafted on the materials surface by ultraviolet (UV) radiation,and the RGD serial obtained by liquid phase synthesis was successfully grafted on the disposed materials by chemical reaction. The endothelialization experiment was made also. The grafting results were measured by X-ray photoelectron spectroscopy (XPS), and endothelialization was observed using optical microscope and scanning electron microscope (SEM). The resutls indicated that the method improves the effect of materials endothelialization. The experiment has made successful use of UV grafting and chemical coupling methods to graft bioactive RGD onto PET film surface. This is a new method of grafting bioactive peptide.

关 键 词：生物活性短肽 RGD PET 表面接枝 生物材料 高分子材料 

分 类 号：R318.08[医药卫生—生物医学工程]