PGLA牙周再生片的细胞相容性研究  

Study on Cytocompatibility of PGLA Film for Periodontal Guided Tissue Regeneration

作　　者：孙皎[1] 何伟[1] 孟爱英[1] 

机构地区：[1]上海第二医科大学附属第九人民医院

出　　处：《生物医学工程学杂志》2003年第3期388-391,共4页Journal of Biomedical Engineering

基　　金：教育部<高等学校骨干教师资助计划>;上海市科技攻关项目(995 2 110 3 8)

摘　　要：研究聚乙交酯 -丙交酯 (PGL A)牙周再生片及其降解产物的细胞相容性。采用不同浸提温度、不同浸提时间和不同浸提比例对 PGL A牙周再生片材料的细胞增殖情况进行实验研究 ;同时用材料在 2、4、6、8、10周时的体外降解液与培养细胞接触 ,观察不同降解周期其降解产物对培养细胞的毒性作用。结果表明 :浸提比例为 0 .1g/ml时 ,37℃下其浸提时间长短对材料的细胞毒性无明显影响 ;浸提比例为 0 .1cm2 /m l时 ,随着浸提温度的升高(5 0℃或 70℃ ) ,材料出现了轻度对细胞毒性反应 ;浸提比例为 0 .5 cm2 /m l时 ,37℃浸提 72 h可引起细胞增殖率下降 ;浸提比例为 6 cm2 /m l时 ,即使是 37℃、2 4 h,也出现了一定程度的细胞毒性。材料浸泡 2、4周 ,其降解产物对细胞无明显的毒性作用 ,材料浸泡后 6周起 ,降解液对细胞相对增殖率作用明显降低。由此提示 :在体外评价试验中 ,试样表面积 (重量 ) /浸提介质的比例大小、浸提温度高低、浸提时间长短以及降解产物在浸提介质中的积聚等因素都可能在一定程度上影响细胞生长。该 PGL A牙周再生片具有良好的细胞相容性。本研究既为 PGL A牙周再生片材料的临床应用提供科学依据 ,又为生物降解类材料的生物安全性评价提供新的研究思路和实验手段。This study was aimed to evaluate the cytocompatibility of PGLA film for periodontal guided tissue regeneration (GTR) and their degradable products. Different extraction temperature, time and ratio were used to assess the cell relative growth rate (RGR) for PGLA. The degradable solution were brought into contact with cultured cells in vitro to observe the effects of cytotoxicity at 2?4?6?8 and 10 weeks. The results showed when the extraction ratio was 0.1g/ml, the extraction time has no effects on cytotoxicity at 37 ℃. When the ratio was 0.1 cm 2/ml, slight cytotoxic reaction appeared with the increase of extraction temperature (50℃ or 70℃). When the extraction ratio was 0.5 cm 2/ml in contract with the degradable solution at 37℃ for 72 h, the cell growth rate decreased. When the ratio was 6 cm 2/ml, the cytotoxicity existed in some degree even if the temperature was at 37℃ for 24h.After 2～4w, the degradable solution had no obvious toxic effects on cells. However, the RGR gradually decreased as the degradation period increased. In conclusion, the effects of the extraction temperature, time, ratio and the degradtion products accumulating in solution on RGR may exist. PGLA film has a good cytocompatibility.

关 键 词：PGLA 牙周再生片 细胞相容性 聚乙交酯-丙交酯 生物降解 细胞毒性 生物学 

分 类 号：R318.08[医药卫生—生物医学工程]