适合多品种番茄RAPD分子标记的引物研究  

Study of Primers to Suit RAPD Markers in Tomato

作　　者：李洪涛[1] 朱海山[1] 张宏[1] 

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201

出　　处：《云南农业大学学报》2003年第4期394-396,共3页Journal of Yunnan Agricultural University

基　　金：云南省科技厅攻关项目(2001NG21)

摘　　要：由于番茄的遗传背景非常狭窄,实验采用两轮引物筛选,利用27个具有代表性的番茄供试材料,从200个引物中筛选出36个PCR扩增效果多态性丰富的引物,克服了以前一些引物在3～4个材料中具有多态性,但它们在用于大量材料的扩增中多态性差的缺点,为RAPD分子标记技术在番茄遗传研究中的广泛应用奠定了基础。Knowing the case that the genetic background of tomato varieties was very narrow, we screened arbitrary primers by 27 varieties and species of tomatoes' RAPD makers for two turns. Among 160 arbitrary primers, We gained 36 primers what possessed abundant polymorphism in the amplification of tomato' DNAs. The result overcame the shortcoming, which primers had polymorphism among 3 or 4 varieties of tomatoes and the polymorphism was not evident most of them in many varieties. It was the basis of the extensive application of RAPD markers in genetic research of tomato.

关 键 词：番茄 RAPD分子标记 遗传研究 PCR 引物 

分 类 号：S641.2[农业科学—蔬菜学]