大鼠原位肝移植模型制作过程中麻醉方法的选择  

Selection of anesthetic method in setting up the animal model of orthotopic liver transplantation in rats

作　　者：彭勇[1] 龚建平[1] 刘长安[1] 王炜[2] 严律南[2] 

机构地区：[1]重庆医科大学附属第二医院肝胆外科,重庆400010 [2]四川大学华西医院普外科,四川成都610041

出　　处：《中国普通外科杂志》2003年第9期673-676,共4页China Journal of General Surgery

基　　金：国家自然科学基金资助项目 (30 2 0 0 2 78) ;重庆市卫生局科研基金资助项目 (渝卫科教 2 0 0 1 - 4 6)

摘　　要：目的　研究在大鼠原位肝移植模型制作过程中麻醉对大鼠的影响 ,以便合理选择正确的麻醉方法。方法　用SD大鼠以改进的二袖套法制作大鼠原位肝移植模型。将 10 0只大鼠随机分为5组 :对照组 ,乙醚组 ,氯胺酮组 ,水合氯醛组 ,戊巴比妥组。观察每种麻醉剂的麻醉过程、对大鼠生理和肝功能的影响以及无肝期开始后的死亡率。结果　每种麻醉剂的麻醉过程不尽相同。麻醉对大鼠生理均有影响 ,其中以乙醚的影响较小 ,戊巴比妥有较大的肝毒性。无肝期开始后氯胺酮组、水合氯醛组、戊巴比妥组死亡率较高 ,而乙醚组无死亡发生。结论　各种麻醉对肝移植大鼠均有明显影响。乙醚具有对生理、肝功影响小 ,无肝期开始后死亡率低的优点 ,可作为制作大鼠原位肝移植模型的首选麻醉方法。Objective To investigate the influences of anesthetic meltod on setting up the animal model of orthotopic liver transplantation(OLT) in rats, in order to properly select the anesthetic method. Methods OLT was performed by modified two-cuff technique in Sprague Dawley rats. 100 rats were randomly divided into 5 groups: control group, ether group, ketamine group, chloral hydrate group and pentobarbital group. The anesthetic process, the influences of anesthetic method on physiologic parameters and hepatic function, and the mortality during anhepatic phase of each group were observed. Results The anesthetic process of each anesthetic method was different,ether anesthesia had fewer impacts on physiologic parameters, and the pentobarbital had great hepatotoxicity. High mortality happened in ketamine group, chloral hydrate group and pentobarbital group during anhepatic phase, while no animal died in ether group. Conclusions Each anesthetic method has significant influences on the OLT rat. Ether anesthesia has fewer effects on physiologic parameters and liver function, and has low mortality during anhepatic phase of OLT rats.So it is the best anesthetic method in setting up the animal model of OLT in rats.

关 键 词：肝移植 疾病模型 动物 麻醉方法 

分 类 号：R657.3[医药卫生—外科学] R363[医药卫生—临床医学]