Study of Tea Digitized Chromatographic Fingerprint Spectra Using Micellar Electrokinetic Chromatography  

作　　者：宋冠群 林金明 屈锋 董文举 

机构地区：[1]Research Center for Eco Environmental Sciences [2]College of Chemistry and Environmental Science

出　　处：《Chinese Journal of Chemistry》2003年第10期1325-1329,共5页中国化学（英文版）

基　　金：ProjectsupportedbytheNationalNaturalScienceFundationforDistinguishedYoungScholarsofChina (No.20125514)andtheNationalNaturalScienceFoundationofChina (Nos .2 0 2 75 0 43and 5 0 2 73 0 46)

摘　　要：This paper described the principle of digitized chromatographic fingerprint spectrum and established digitized chromatographic fingerprint spectra of ten brands of Chinese famous tea by the micellar electrokinetic chromatography. This work was done using a 25 mmol·L -1 sodium dodecylsulfate in a 20 mmol·L -1 borate (pH 7 0) solution as running buffer, 20 kV applied potential and detection at 280 nm. The chromatographic fingerprint spectra were digitized by the relative retention value ( α ) and the relative area ( S r), and were analyzed to identify the tea samples. In the absence of the standard samples, the present method was easy setup and inexpensive, and provided the applicable information for the quality assessment of teas.This paper described the principle of digitized chromatographic fingerprint spectrum and established digitized chromatographic fingerprint spectra of ten brands of Chinese famous tea by the micellar electrokinetic chromatography. This work was done using a 25 mmol·L -1 sodium dodecylsulfate in a 20 mmol·L -1 borate (pH 7 0) solution as running buffer, 20 kV applied potential and detection at 280 nm. The chromatographic fingerprint spectra were digitized by the relative retention value ( α ) and the relative area ( S r), and were analyzed to identify the tea samples. In the absence of the standard samples, the present method was easy setup and inexpensive, and provided the applicable information for the quality assessment of teas.

关 键 词：tealeaves micellar electrokinetic chromatography digitized chromatographic fingerprint spectrum quality assessment 

分 类 号：O658[理学—分析化学]