栉孔扇贝的急性病毒性坏死症(AVND)病毒多克隆抗体的制备及ELISA分析  

Preparation of Polyclonal Antibody of AVND Virus and Analysis by ELISA Technique

作　　者：王秀华[1] 贺桂珍[2] 李赟[2] 王崇明[1] 宋微波[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]中国海洋大学海水养殖教育部重点实验室,青岛266003

出　　处：《高技术通讯》2003年第9期84-88,共5页Chinese High Technology Letters

基　　金：973规划(G1999012001)。

摘　　要：以青岛太平角养殖海区患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为抗原,制备出效价较高的多克隆抗体。用酶联免疫吸附分析(ELISA)方法对青岛黄岛等不同养殖海区、不同时间采集的栉孔扇贝材料进行检测,结果表明,7、8月份样品检测结果呈强阳性,检出阳性率为75％～95％,其他时期阳性率较低。The acute virus necrobiotic disease virus(AVND virus)was isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. Indirect ELISA technique was set up and was used to detect the AVND virus within 5 to 7 hours, which the minimum quantity of virus to be detected was 30ng. Then scallop specimens from different sampling sites in various periods were detected by ELISA technique. The results showed that the viral infection rate was 75% -95% during epizootic period in July and August and was low in other growth period.

关 键 词：栉孔扇贝 急性病毒性坏死症病毒 多克隆抗体 ELISA 制备方法 酶联免疫吸附分析 阳性率 

分 类 号：S944[农业科学—水产养殖]