嗅神经鞘细胞条件培养液对PC12细胞活性及分化影响  

Effects of Olfactory Ensheathing Cell Conditioned Medium on the Survival and Differentiation of PC12 Cells

作　　者：杨浩[1] 于玲[1] 王春婷[1] 李静文[1] 鞠躬[1] 

机构地区：[1]第四军医大学全军神经科学研究所,陕西西安710032

出　　处：《动物医学进展》2003年第5期65-67,118,共4页Progress In Veterinary Medicine

摘　　要：研究嗅神经鞘细胞 (OEC)条件培养液对 PC1 2细胞活性和分化的影响。原代培养并纯化 OEC,收集其培养上清并制备条件培养液 ,然后用其培养 PC1 2细胞 ,随后于倒置相差显微镜下观察细胞形态学变化 ,通过 MTT检测 PC1 2细胞活性 ,免疫细胞染色观察β-tubulin的表达变化。结果显示 OEC条件培养液可促进 PC1 2细胞突起生长 ,增加细胞活性 ,促进其 β-tubulin的表达升高。从而证明 OEC能够分泌一些促进PC1The present study was aimed to investigate whether adult olfactory ensheathing cell(OEC) could release some bioactive factors to affect the survival and differentiation of PC12 cells. OEC was cultured and purified,and the cell conditioned medium was harvested and used to culture PC12 cells. Morphological change was observed under invert phase contrast microscope, cell viability was evaluated via MTT, Expression of β tubulin was examined by means of immunocytochemistry, eventually, these positive cells were calculated. Results showed OEC conditioned medium can induce neurite outgrowth from PC12 cells, and enhance the cell viability, upregulate the expression of β tubulin. These results suggested that olfactory ensheathing cell secrete some factors in vitro to promote the survival and differentiation of PC12 cells.

关 键 词：嗅神经鞘细胞 OEC 条件培养液 PC12细胞 活性 分化 细胞形态学 牛 

分 类 号：S858.23[农业科学—临床兽医学]