芽孢杆菌B_(11)拮抗蛋白性质及其对西瓜枯萎病菌的作用机理  

Characterization of antagonistic protein from Bacillus sp. B_(11) and its mechanism against Fusarium oxysporum on water melon

作　　者：彭好文[1] 黎起秦[1] 蒙姣荣[1] 唐照磊[1] 林纬[1] 

机构地区：[1]广西大学农学院,南宁530005

出　　处：《植物保护》2003年第5期22-25,共4页Plant Protection

基　　金：广西自然科学基金项目 (桂科基 0 0 0 90 18)

摘　　要：芽孢杆菌 (Bacillussp .)B11分离自土壤 ,其拮抗蛋白粗提液在温度 6 0、80℃各处理 30min后仍能保持抗菌活性 ,对紫外线、蛋白酶K、RNase处理稳定 ,对胃蛋白酶、胰蛋白酶、氯仿部分敏感 ,作用活性 pH (pH 4～ 10 )范围较宽。B11拮抗蛋白对西瓜枯萎病菌具有较强的拮抗作用 ,其抑菌机制主要使病菌菌丝畸形 ,细胞质浓缩 。An antagonistic bacteria strain B 11 , Bacillus sp., isolated from the soils, strongly inhibits the growth of Fusarium oxysporum f. sp. niveum. A crude protein produced by B 11 tolerates high temperature (60℃ and 80℃), ultraviolet rays, proteinase K and RNase, but it is sensitive to pepsin, trypsin and chloroform. The active pH range of crude protein to the pathogen is wide, from 4.0 to 10.0. The mechanism of antagonistic protein against the growth of F. oxysporum was mainly to make hypha abnormal, cytoplasm condensation, and the spores germinate abnormally.

关 键 词：芽孢杆菌 拮抗蛋白 性质 西瓜 枯萎病菌 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]