Electrochemical Metalloimmunoassay Based on Enlargement of Gold Nanoparticle Label Treated with Ag Enhancement System  

作　　者：WANGMei-jia YUANHang JIXiao-hui LIUZhao-yue JIARuo-kun WANGLian-ying LIUYan-mei MALan LIJing-hong BAIYu-bai LITie-jin 

机构地区：[1]CollegeofChemistry,JilinUniversity,Changchun130021,P.R.China [2]KunmingYundaMonoclonalAntibodyTechnologyCenter,YunnanUniversityKunming650091,P.R.China [3]StateKeyLaboratoryofElectroanalyticalChemistry,ChangchunInstituteofAppliedChemistry,ChineseAcademyofSciences,Changchun130022,China

出　　处：《Chemical Research in Chinese Universities》2003年第4期413-416,共4页高等学校化学研究（英文版）

基　　金：Supportted by the National Natural Science Foundation of China( No.2 0 0 730 16 ,6 0 2 710 2 0 ),86 3projects( No.2 0 0 2 AA302203)and973projects( No.G19990 6 4 5 0 4 )

摘　　要：A novel sensitive electrochemical immunoassay with colloidal gold as the antibody labeling tag and subsequent signal amplification by silver enhancement is described. Colloidal gold was treated by a light-sensitive silver enhancement system which made silver deposit on the surface of colloidal gold(form Au/Ag core-shell structure), followed by the release of the metallic silver atoms anchored on the antibody by oxidative dissolution of them in an acidic solution and the indirect determination of the dissolved Ag + ions by anodic stripping voltammetry(ASV) at a carbon fiber microelectrode. The electrochemical signal is directly proportional to the amount of analyte(goat IgG) in the standard or a sample. The method was evaluated by means of a noncompetitive heterogeneous immunoassay of immunoglobulin G(IgG) with a concentration as low as 0.2 ng/mL. The high performance of the method is related to the sensitive ASV determination of silver(Ⅰ) at a carbon fiber microelectrode and to the release of a large number of Ag + ions from each silver shell anchored on the analyte(goat IgG).A novel sensitive electrochemical immunoassay with colloidal gold as the antibody labeling tag and subsequent signal amplification by silver enhancement is described. Colloidal gold was treated by a light-sensitive silver enhancement system which made silver deposit on the surface of colloidal gold(form Au/Ag core-shell structure), followed by the release of the metallic silver atoms anchored on the antibody by oxidative dissolution of them in an acidic solution and the indirect determination of the dissolved Ag + ions by anodic stripping voltammetry(ASV) at a carbon fiber microelectrode. The electrochemical signal is directly proportional to the amount of analyte(goat IgG) in the standard or a sample. The method was evaluated by means of a noncompetitive heterogeneous immunoassay of immunoglobulin G(IgG) with a concentration as low as 0.2 ng/mL. The high performance of the method is related to the sensitive ASV determination of silver(Ⅰ) at a carbon fiber microelectrode and to the release of a large number of Ag + ions from each silver shell anchored on the analyte(goat IgG).

关 键 词：Colloidal gold Silver enhancement Anodic stripping voltammetry(ASV) 

分 类 号：O614.122[理学—无机化学] O657.1[理学—化学]