5′—磷酸尿苷酸的阴极溶出伏安特性  

INVESTIGATIONS ON BIOANALYTICAL CHEMISTRY PART Ⅳ.CATHODIC STRIPPING VOLTAMMETRY OF URACIL 5'-MONOPHOSPHATE

作　　者：金文睿[1] 赵昕[1] 温新欣[2] 汪复宁 高祖泉 

机构地区：[1]山东大学化学系 [2]山东大学生物系

出　　处：《山东大学学报（自然科学版）》1992年第4期490-494,共5页Journal of Shandong University(Natural Science Edition)

摘　　要：研究了pH=9.68的硼砂—NaOH缓冲底液中5′-磷酸尿苷酸(5′-UMP)在悬汞电极上的阴极溶出伏安行为,讨论了富集电势、富集时间对伏安曲线的影响及最佳测定条件,方法的检测下限为5×10^(-10)mol/L.The cathodic stripping voltammetric behaviour of uracil 5'-monophosphate(5'-UMP)at a hanging mercury drop electrode has been investigated in Na_2B_4O_7-NaOH buffer.Theinfluences of the pre-concentration potential and pre-coneentration time on the peak currentand peak potential,as well as the optimum experimental conditions are discussed.The linearrelationship between the peak current and the concentration of 5'-UMP at different pre-concentration potentials are tested.The detection limit of 5'-UMP is 5×10^(-10)mol/L.Therelative standard deviation of the method is 7.2% for 1×10^(-7)mol/L 5'-UMP.

关 键 词：溶出伏安法 磷酸尿苷酸 核酸 

分 类 号：Q52[生物学—生物化学]