密蒙花(Buddlija officinalis)基因转化受体系统的建立  

The Callus Induction Culture of Buddleja Officianlis

作　　者：白洁[1] 刘将新[1] 唐琳[1] 祝欣[1] 刘文娟[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2003年第5期958-962,共5页Journal of Sichuan University(Natural Science Edition)

摘　　要：以密蒙花(BuddlijaofficinalisMaxim)芽、叶、茎段为外植体,在B5,MS,White培养基上获得愈伤组织.结果表明:芽、幼叶脱分化能力差异不大;不同时期的叶片差异较大,以幼叶诱导为佳;作者筛选出的愈伤组织最佳培养基为B5+6 BA0.5mg/L+2,4 D0.5mg/L.在继代培养阶段,B5比MS和White更适合细胞的快速生长和繁殖;并且以叶片的愈伤组织生长较快,芽、茎段次之.最佳继代培养基为B5+6 BA0.5mg/L+NAA0.5mg/L.继代的愈伤组织可在B5+6 BA1mg/L+NAA0.1mg/L+GA0.1mg/L培养基中分化出幼苗,在生根培养基1/2MS+NAA0.2mg/L或1/2MS+NAA0.6mg/L中分化出根且获得再生植株.将长4～6cm的再生小苗练苗3d后移植,幼苗成活率达100%,并于当年开花.The callus is initiated in three media (B5,MS,White) from the buds, leaves and stem segments of Buddleja officinalis. The results shows that the inducing frequencies of tender leaves of Buddleja officinales on media are as high as 100%. No significant differences between tender leaves and buds is observed, as to the frequency. The frequency was decreased as the leaf became older. The medium with 6BA 0.5mg/L and NAA 1.0mg/L is selected as the best medium for callus culture. However, B5 is more suitable for calli and cells to grow and divide than MS and White. The growth rate of leaves is faster than buds and stem segments. The Medium with 6BA 0.5mg/L and NAA 1.0mg/L is applicable to increase the cell growth. The medium with 6BA 0.5mg/L and NAA 0.5mg/L is applicable to subculture. The B5 midium containing 6BA 1mg/L, NAA 0.1mg/L and GA 0.1mg/L was favorable to the regeneration of the calli from subculture,and the growth of roots is best on 1/2 MS media containing NAA 0.2mg/L or NAA 0.6 mg/L. Seedlings with growing to 4～6cm were transplanted to the pots with disinfect soil after 3 days training. The survival rate of transplanted plantlet reached 100%. So, two gene transformation systems of Buddleja officinales were established. Callus induced from explants of Buddleja officinales are suitable for Biolistical gun transformation and for Agrobacteriummediated transformation.

关 键 词：密蒙花 愈伤组织 继代培养 植株再生 基因转化系统 

分 类 号：Q943[生物学—植物学]