猪瘟弱毒苗预防牛病毒性腹泻/粘膜病的短期安全性与微量中和试验  

The Microculture Neutralization Test and the Stort Period Security Test of Prevention Bovine Viral Diarrhoea - Mucosal Disease with Attenuated Vaccine of Hog Cholera Virus

作　　者：刘亚刚[1] 殷中琼[1] 刘世贵[1] 韩鹏[2] 华莎[2] 余勇[3] 石谦[3] 丁晓涛[4] 于若飞[5] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]西南民族大学,成都610064 [3]四川省动物防疫监督总站,成都610041 [4]甘孜州畜牧业科学研究所,四川康定626000 [5]农业部成都生物药械厂,成都610066

出　　处：《四川大学学报（自然科学版）》2003年第5期970-973,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省畜牧食品局"十五"规划整治重点项目基金

摘　　要：作者用猪瘟弱毒苗预防牛病毒性腹泻/粘膜病的短期安全性和中和抗体效价进行了测定.动物短期安全性试验结果表明:用大剂量(每头10～25个免疫剂量单位)猪瘟弱毒苗注射犊牛、成年奶牛、怀孕母牛和牦牛后,对体温、食欲、生产性能及妊娠牛的胎儿均无不良影响.微量中和试验结果表明:成年奶牛每头注射6个免疫剂量单位的猪瘟弱毒苗15d后,有50%产生保护性中和抗体;每头注射10个免疫剂量单位的猪瘟弱毒苗15d后,有83.3%产生保护性中和抗体;小牛每头注射3个免疫剂量单位的猪瘟弱毒苗15d后,有50%产生保护性中和抗体;抗体产生的滴度与免疫剂量成正比.上述结果表明用猪瘟弱毒苗预防牛病毒性腹泻/粘膜病是安全有效的,具有实用价值.The safety and immunity of the attenuated HCV vaccine was injected into cattle to prevent Bovine viral Diarrhoea were reported.The results of short period security test as follow: After being injected big dosage (10～25 times dosage) of attenuated HCV vaccine, the body temperature, food appetite, production function and the fetus of pregnancy cow of the calf, milch cow, pregnancy cow and the yak are free from side and toxicity effect. The results of microculture neutralizetion test as follow: the rate of protective neutralizing antibody (1∶11.8) is 50%, when each milch cows was injected 6 times dose of HCV vaccine after 15d,the rate is 50%, when each calf was injected 3 times dose of HCV vaccine after 15 d. From this, the titer and vecocity of BVD neutralizing antibody is in parallel with the dose of vaccine HCV injected. Conclusion, study showed that the vaccine of HCV injection into cattle to prevent BVD/MD is innocent safe and effective.

关 键 词：猪瘟弱毒苗 短期安全性试验 微量中和试验 牛病毒性腹泻/粘膜病 

分 类 号：S852.65[农业科学—基础兽医学]