新型荧光试剂用于小肽的毛细管电泳分离  

SEPARATION OF OLIGOPEPTIDES BY CAPILLARY ELECTR OPHORES IS USING 9-(2-CARBAZOLE)ETHYL CHLOROFORMATE(CEOC) AS DERIVATIZATION AGENT

作　　者：明永飞[1] 谢怡[1] 孙玉希[1] 石运伟[1] 尤进茂[1] 

机构地区：[1]曲阜师范大学化学科学学院,山东省曲阜市273165

出　　处：《曲阜师范大学学报（自然科学版）》2003年第4期73-76,共4页Journal of Qufu Normal University(Natural Science)

基　　金：国家自然科学基金 (2 0 0 75 0 16)资助项目

摘　　要：采用新合成的荧光试剂咔唑_9_乙基氯甲酸酯 (CEOC)作为柱前衍生试剂 ,在胶束电动色谱的模式下考察并优化了小肽类衍生物分离的关键条件 ,实现了 5种小肽在 6min内的快速基线分离 .在选定条件下 ,对其线性范围、检测限和重现性进行了测定 .结果表明 ,所建立的方法简单快速 ,肽的分离度均达到 5 .A simple and rapid method for the separation of olig opeptides by micellar electrokinetic chromatography using 9_(2_carbazole)ethyl c hloroformate (CEOC) as derivatization agent was developed. The effect of some ke y factors on the separation of oligopeptide derivatives was investigated, and fi ve oligopeptide derivatives were separated with satisfactory results in 6 min. The linear range, the limit of detection and the repeatability were investigated under the selected conditions. The resolution of five oligopeptide derivatives was above 5.0.

关 键 词：小肽 毛细管电泳 分离方法 荧光试剂 胶束电动色谱 咔唑-9-乙基氯甲酸酯 衍生化 

分 类 号：Q516.03[生物学—生物化学]