假丝酵母类金属硫蛋白的分离、纯化及功能研究  

STUDY ON ISOLATION, PURIFICATION AND FUNCTION OF METALLOTHIONEIN-LIKE PROTEIN FROM CANDIDA SP.

作　　者：李明春[1] 杨炤[1] 张飙[2] 邢来君[1] 

机构地区：[1]南开大学微生物学系,天津300071 [2]天津中医学院,天津300193

出　　处：《南开大学学报（自然科学版）》2003年第3期123-128,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：假丝酵母Cu-12(Candida sp.Cu-12)经Cu2+诱导产生的蛋白进行Sephadex G-100和纤维素DE-52 分离纯化及理化性质测定,获得的蛋白具有四个亚型,其主理化性质均与MT的定义相符,因此确定得到了假丝 酵母的类金属硫蛋白,同时测定了该Cu-MT清除羟自由基和氧自由基的能力.The protein was produced from Candida sp. Cu-12 induced with CuCl2, isolated by means of Sephadex G-100 and DEAE-cellulosecolumn chroma tography, and determined physicochemical characteristics. Four types of this protein were found and most physicochemi-cal characteristics were in line with the definition of metallothionein. So those proteins were metallothionein-like protein of Candida sp. Meanwhile, the ability of Cu-MTs to scavenge hy-droxyl radical and oxygen radical was determined.

关 键 词：类金属硫蛋白 假丝酵母 分离纯化 自由基 

分 类 号：TQ93[化学工程]