意蜂工蜂酸性磷酸酶的化学修饰及荧光光谱  

Chemical modification and fluorescence spectrum of acid phosphatase from APIS CERANA

作　　者：江琰[1] 刘克武[1] 

机构地区：[1]四川大学生命科学学院,四川成都610064

出　　处：《化学研究与应用》2003年第5期658-660,共3页Chemical Research and Application

摘　　要：本文对意蜂工蜂酸性磷酸酶(ACPase,E.C.3.1.3.2)在分离纯化的基础上,研究化学修饰剂苯甲基磺酰氟(PMSF)、二巯基苏糖醇(DTT)、对氯汞苯甲酸(pCMB)、N 溴代琥珀酰亚胺(NBS)、溴代乙酸(BrAc)在不同浓度,不同时间条件下对酶活力及荧光光谱的影响。结果表明:半胱氨酸残基的巯基和二硫键被修饰后,酶活力下降且荧光强度减少;色氨酸残基被修饰后酶活力降低,荧光强度减小;含羟基的氨基酸残基被修饰后酶活力降低,荧光强度增加;组氨酸残基被修饰后酶活力基本不变,酶的荧光强度也不改变。Acid Phosphatase(ACPase)was isolated and purified from APIS CERANA.The effects of different modification reagents on the ACPase catalytic activity and its fluorescence spectra were determined.The result indicated that when the tryptophane residue of ACPase,and sulfhydryl group and disulfide link of cysteine residue were modified,the related activity of the enzyme was decreased and its fluorescence emission peak intensity was reduced;when the amino acids with hydroxyl group were modified,the related activity of the enzyme was inhibited and the fluorescence emission peak intensity was increased.Modification of histidine residue did not change the activity of enzyme and its fluorescence emission peak intensity.

关 键 词：意蜂工蜂 酸性磷酸酶 化学修饰 荧光光谱 

分 类 号：Q555.7[生物学—生物化学] O657.34[理学—分析化学]