何首乌茎段离体培养的研究  

A STUDY ON THE STEM SEGMENTS CULTURE IN VITRO OF POLYGONUM MULTIFLORUM THUNB

作　　者：李娟玲[1] 刘国民[1] 邱文华[1] 徐立新[1] 

机构地区：[1]海南大学农学院,海口570228

出　　处：《贵州科学》2003年第3期86-91,共6页Guizhou Science

摘　　要：　用何首乌种子经表面消毒后进行无菌发芽并建立了无菌材料,以无菌实生苗茎段作外植体进行离体快繁研究。试验了不同基本培养基(MS,H,1/2MS,B5和N6),不同种类外源激素(IBA、NAA、KT)和不同浓度梯度的外源激素(0 0mg/L,0 1mg/L,0 5mg/L,1 0mg/L,2 0mg/L和4 0mg/L)以及不同蔗糖浓度(0 0%,1 0%,2 0%,4 0%,8 0%,16 0%)对茎段离体培养的影响。结果表明:MS基本培养基,0 5mg/LIBA(或0 5mg/LNAA)及3%的蔗糖比较适合于何首乌的茎段离体快繁。推荐用于何首乌茎段离体快繁的最佳培养基配方为:MS+肌醇100mg/L+盐酸硫胺素1 0mg/L+盐酸吡哆素0 5mg/L+甘氨酸2 0mg/L+IBA0 5mg/L(或NAA0 5mg/L)+蔗糖3 0%+琼脂0 75%,pH5 9。用该培养基对何首乌进行茎段离体快繁,每次继代培养可增殖4～5倍。This paper deals with the stem segment cultures in vitro of Polygonum muliflorum Thunb.. The seeds of P. multiflorum, treated via surface sterilization, were used for the sterile germination to establish the sterile materials. The stem segments of the sterile seedlings were used as the explants to study the rapid propagation in vitro. Different basic media (MS,H,1/2MS,B5 and N6), different kinds of hormenes (IBA, IAA and KT), different concentrations of the exogenous hormenes above (00mg/L,01mg/L,05mg/L,10mg/L,20mg/L and 40mg/L), and different sucrose concentrations (00%,10%,20%,40%,80% and 160%) wrer tested to determine the effects on the stem segment culture in vitro of P. multiflorum. The experimental results showed that basic medium MS, 05mg/L NAA and 3% sucrose were fairly suitable for the rapid propagation of P. multiflorum via stem segment culture in vitro. A prescription of the optimal medium for the rapid propagation of P. multiflorum Via stem segment culture in vitro was 'MS + 100mg/L inostol +05mg/L nicotinic acid +05mg/L pryidoxine·HCl+10mg/L thiamine · HCl+20mg/L glycine+05mg/L IBA (or:05mg/L NAA)+30% sucroce +075% agar, pH 59'. A propagation coefficient of 4～5 could be obtained by using the recommended medium prescription above.

关 键 词：何首乌 茎段 离体培养 体速快繁 培养基 继代培养 良种选育 中药 

分 类 号：S567.239[农业科学—中草药栽培] S336[农业科学—作物学]