提取时间、溶剂对丹参提取物质量的影响研究  

Study of effect of extracting time and solvent on the quality of extract of Salvia Miltiorrhiza Bunge

作　　者：倪力军[1] 史晓浩 高秀蛟[1] 王宁 

机构地区：[1]华东理工大学,上海200237 [2]上海市雷允上药业有限公司,上海200001

出　　处：《中成药》2003年第10期780-782,共3页Chinese Traditional Patent Medicine

基　　金：国家十五攻关项目 (项目编号 2 0 0 1BA70 1A0 4)

摘　　要：目的 :研究提取时间、溶剂对丹参中主要活性成分含量的影响 ,为GEP建设提供基础数据和理论指导。方法 :用HPLC在不同提取时间下分别测定丹参 90 %乙醇提取液和水提取液中丹酚酸B和丹参酮ⅡA的含量。结果 :提取时间和提取溶剂对丹参提取液中丹酚酸B和丹参酮ⅡA的含量影响显著。结论 :丹参中丹酚酸类成分宜用水提取 ,提取的时间为 4 0～ 5 0min ;丹参酮类成分宜用乙醇提取 ,时间为 2 0～ 30min。Objective:To study the effect of the extracting time and solvent on the concentration of main active components in Salvia Miltiorrhiza Bunge, and to provide basic data and theoretical guide to GEP(general extraction practice). Methods: Salvianolic acid B and Tanshinone ⅡA were extracted by 90% alcohol and water respectively from Salvia Miltiorrhiza Bunge and their contents were etermined by means of HPLC at regular intervals. Results: Extracting time and solvent were significant factors in the prcess of concentrating Salvianolic acid B and Tanshinone ⅡA from extract of Salvia Miltiorrhiza Bunge. Conclusion: Water is suitable for extracting Salvianolic acids, the extracting time is 40～50 minutes; alcohol is suitable for extracting Tanshinones, the extracting time is 20～30 minutes.

关 键 词：GEP 丹酚酸B 丹参酮ⅡA 提取 

分 类 号：R284.2[医药卫生—中药学]