钙和钙离子载体A23187对水稻早期胚胎离体发育的影响  

Effects of Calcium and Ionophore A23187 on in vitro Development of Early Embryo in Rice (Oryza sativa L.)

作　　者：吴娟子[1] 赵洁[1] 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室武汉大学生命科学学院,武汉430072

出　　处：《武汉植物学研究》2003年第5期375-384,共10页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金项目(30170091);国家重大基础研究前期专项项目(2002CCA00100)资助。

摘　　要：研究了不同浓度的钙(Ca2+)和钙离子载体A23187对水稻早期胚胎离体发育的影响。结果表明:(1)Ca2+对授粉后3～5d水稻胚胎离体发育的调控具有时间和浓度效应。培养基中不含Ca2+或Ca2+浓度较高(10-1mol/L)时,3d原胚离体分裂和生长完全受到抑制;4～5d早期分化胚受到一定程度的影响;而Ca2+浓度为10-3mol/L时,不同时期的水稻胚胎均表现出最佳的生长速度和最高的离体胚胎发生频率;在相同的钙浓度条件下,胚龄越大,胚胎发生频率及总诱导频率越大。(2)A23187影响水稻胚胎的离体生长和形态发生:胚胎越小,影响越大;浓度越高,抑制作用越强。The effects of calcium and ionophore A23187 on in vitro development of early embryo in rice(Oryza sativa L.)were studied.The main results were as follows:(1) the influence of Ca2+ on the in vitro development of 3 to 5dayold embryos was related to the concentration of Ca2+ and the age of embryos.Without Ca2+ or with the high concentration of Ca2+(10-1 mol/L),the growth of 3dayold embryos was inhibited completely and the development of 4 to 5dayold embryos was affected in certain degree.With 10-3 mol/L Ca2+,3 to 5dayold embryos grew rapidly and the highest frequency of embryogenesis appeared.In the same concentration of Ca2+,the older the embryos were,the higher the frequency of embryogenesis and the total induction appeared.(2) A23187 influenced in vitro embryo development and morphosis.With increasing A23187 concentration,the in vitro development of the embryos in rice was inhibited more violently.The younger embryos were,the more obvious inhibition.

关 键 词：水稻 CA^2+ Ca^2+载体A23187 早期胚胎 离体发育 

分 类 号：Q944.46[生物学—植物学] S511[农业科学—作物学]