细菌质粒DNA的快速提取及检测  

A Simple and Rapid Method for Extraction of Plasmid DNA and its Detection

作　　者：李亮[1] 蔡志强[1] 赵希岳[1] 

机构地区：[1]江苏工业学院生物工程实验室,江苏常州213016

出　　处：《江苏工业学院学报》2003年第3期30-31,共2页Journal of Jiangsu Polytechnic University

摘　　要：介绍一种质粒DNA的快速提取方法,所分离出的DNA纯度较好,全部操作仅在两只Eppendorf管中进行,含质粒DNA的上清液可直接点样走琼脂糖凝胶电泳。整个提取过程在较短时间内完成。该方法简便、快捷、效果好。This paper introduced a rapid method for extraction of plasmid DNA at a higy purity.All the process could be completed only in two Eppendorf tubes.The supernatant liquid containing plasmid DNA could be directly sampled for agarose gel electrophoresis.The entire procedure could be finished in short time.This method was rapid,simple.

关 键 词：细菌质粒DNA 快速提取 检测 凝胶电泳 

分 类 号：Q75[生物学—分子生物学]