雌激素拮抗剂ICI-182780对ZDY101调节CHOm_2细胞M_2受体密度的影响  

The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M_2 receptor density in CHOm_2 cells

作　　者：王雪松[1] 胡雅儿[1] 夏宗勤[1] 

机构地区：[1]上海第二医科大学细胞调控研究室,上海200025

出　　处：《核技术》2003年第11期845-848,共4页Nuclear Techniques

基　　金：国家自然科学基金(30070926)资助

摘　　要：CHOm2细胞加雌二醇、ZDY101或等体积溶媒DMSO培养后通过3H-NMS结合反应测定M受体密度,并与同时加雌激素受体拮抗剂ICI-182780的细胞作对比。结果显示:雌二醇组M受体密度为(123.7±23.9)fmol·mg-1,加ICI-182780后为(84.8±19.8)fmol·mg-1;相应的DMSO对照组为(89.6±22.9)fmol·mg-1,加ICI后为(66.9±22.5)fmol·mg-1;雌二醇组加ICI-182780后M受体密度降低的幅度远大于DMSO对照组。ZDY101组M受体密度为(147.3±36.9)fmol·mg-1,加ICI-182780后为(120.8±40.4)fmol·mg-1;相应的DMSO对照组为(95.9±24.0)fmol·mg-1,加ICI-182780后为(77.0±22.3)fmol·mg-1。表明雌激素和ZDY101都具有的提高M受体密度的功效,雌激素的药效可被ICI抑制,而ZDY101的药效不被ICI抑制,说明ZDY101有不同于雌激素的作用机制。After the treatment of estradiol, ZDYIOI or DMSO (solvent) to cultured CHOm2 cells for a certain peirod, M-receptor density was determined by 3H-NMS binding assay. Parallel observations were carried out with or without the addition of ICI-182780. The Results show that the density of M receptor in cells treated with estradiol or DMSO control were 123.7±23.9 and 89.6±22.9fmol/mg protein, and these values dropped down to 84.8±19.8 and 66.9±22.5fmol/mg with the presence of ICI-182780. The density of M receptor in cells treated by ZDYIOI or DMSO control were 147.3±36.9 and 95.9±24.0fmol/mg, and dropped to 120.8±40.4 and 77.0±22.3fmol/mg protein with the presence of ICI-182780. ICI-182780 caused a much greater decrease of M receptor density in estradiol treated cells than DMSO control cells indicating that estradioi increased the M receptors through action on estradiol receptor. The magnitude of decrease of M receptor density induced by ICI-182780 in ZDYIOI treated group was similar to that in DMSO controls, indicating that the increase of M receptor density induced by ZDY101 could not be inhibited by ICI-182780 and hence had a mechanism different from estradiol.

关 键 词：M受体 阿尔采默病 ZDY101 知母 雌二醇 ICI-182780 

分 类 号：R817.1[医药卫生—影像医学与核医学]