镍化合物诱发恶性转化细胞的端粒酶活性表达  

TELOMERASE ACTIVITY IN THE MALIGNANT PROCESS OF CELLS INDUCED BY NICKEL COMPOUNDS

作　　者：王敏[1] 吴中亮[1] 陈家堃[1] 

机构地区：[1]广州医学院化学致癌研究所,广东广州510182

出　　处：《癌变．畸变．突变》2003年第4期193-195,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助(No:39170651)

摘　　要：目的 :探讨二种镍化合物诱发细胞恶变过程中端粒酶活性的变化。方法 :二种镍化合物转化细胞接种BALB/c裸鼠 ,应用端粒重复序列扩增法(telomericrepeatamplificationprotocol,TRAP)检测转化细胞和肿瘤细胞的端粒酶活性。 结果 :结晶型硫化镍和氯化镍诱发的BALB/c-3T3恶性转化细胞的端粒酶活性均表现为阳性。结论 :镍化合物诱发的细胞恶性转化涉及了端粒酶活性改变 。Purpose: To explore the telomerase activity in the malignant process of BALB/c-3T3 cells induced by crystalline nickel sulfide, nickel chloride. Methods: TRAP-sliver staining assay was used to detect the telomerase activity. Results: Telomerase activity in BALB/c-3T3 cells transformed by nickel sulfide and nickel chloride were all positive. Conclusion: The positive expression of telomerase activity in the cell transformation might be related to the nickel carcinogenesis.[

关 键 词：镍化合物 转化细胞 肿瘤细胞 端粒酶 

分 类 号：R135.1[医药卫生—劳动卫生] R730.2[医药卫生—公共卫生与预防医学]