顺铂、卡铂和双环铂对pBR322质粒DNA的断裂作用  

THE BREAKING EFFECTS OF CISPLATIN, CARBOPLATIN AND DICYCLOPLATIN ON pBR322 PLASMID DNA

作　　者：马华智[1] 刘正兴[1] 王全军[1] 阎长会[1] 廖明阳[1] 

机构地区：[1]军事医学科学院毒物药物研究所四室,北京100850

出　　处：《癌变．畸变．突变》2003年第4期220-222,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的 :研究铂类抗癌药顺铂、卡铂和双环铂对 pBR322质粒DNA的致断性。 方法 :用琼脂糖凝胶电泳和凝胶成像分析方法结果 :顺铂、卡铂、双环铂均可诱发 pBR322质粒DNA断裂 ,对质粒DNA的半数致断剂量分别为33.71μmol/L、3.31mmol/L和1.61mmol/L。 结论 :对pBR322质粒DNA的致断性强弱依次为 :顺铂>双环铂>Purpose:To study the breaking effects of Cisplatin, Carboplatin and Dicycloplatin on pBR322 plasmid DNA. Methods:Agrose gel electrophoresis and Gel image analysis was used. Results:All three platinum-species drugs could cause DNA break of pBR322 plasmid. The FC50 of Cisplatin, Carboplatin and Dicycloplatin were 33.71 μmol/L, 3.31 mmol/L, and 1.61 mmol/L , respectively. Conclusion:Breaking effects of tested drugs: Cisplatin > Dicycloplatin > Carboplatin.

关 键 词：顺铂 卡铂 双环铂 DNA断裂 琼脂糖凝胶电泳 凝胶成像分析 

分 类 号：R979.1[医药卫生—药品] R994[医药卫生—药学]